| Sherlock MIS系统应用于土壤细菌鉴定的研究 |
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| 引用本文： | 吴愉萍,徐建明,汪海珍,胡宝兰,吴建军.Sherlock MIS系统应用于土壤细菌鉴定的研究[J].土壤学报,2006,43(4):642-647. |
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| 作者姓名： | 吴愉萍 徐建明 汪海珍 胡宝兰 吴建军 |
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| 作者单位： | 浙江大学土水资源与环境研究所,杭州,310029 |
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| 基金项目： | 国家杰出青年科学基金;国家自然科学基金;国家重点基础研究发展计划(973计划) |
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| 摘 要： | 以10种已知菌株为例，对sherlock微生物鉴定系统（Sherlock MIS）的细菌鉴定准确性及培养基、活化时间和取样区域等因素对鉴定结果的影响进行了研究。结果表明该系统对TSBA培养基培养的细菌的鉴定准确率很高，除苏云金杆菌外其他菌株均鉴定到种的水平；但选用牛肉膏蛋白胨培养基培养细菌后，系统对供试菌株鉴定效果不理想。不同活化处理中所有样品的鉴定结果均正确，但以菌种活化2次后的SI值（相似指数）更高；取菌在二区或是三区则对鉴定结果影响不大。
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| 关 键 词： | 细菌鉴定 磷脂脂肪酸 |
| 收稿时间： | 2005-11-30 |
| 修稿时间： | 2005-11-302006-02-27 |
APPLICATION OF SHERLOCK MIS IN IDENTIFICATION OF SOIL BACTERIA |
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| Wu Yuping,Xu Jianming,Wang Haizhen,Hu Baolan and Wu Jianjun.APPLICATION OF SHERLOCK MIS IN IDENTIFICATION OF SOIL BACTERIA[J].Acta Pedologica Sinica,2006,43(4):642-647. |
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| Authors: | Wu Yuping Xu Jianming Wang Haizhen Hu Baolan and Wu Jianjun |
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| Institution: | Institute of Soil and Water Resources and Environmental Science, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | Ten known strains of bacteria were used to study validity of the Sherlock Microbial Identification System(Sherlock MIS) in identification of soil bacteria.Results show that Sherlock MIS is accurate in identifying all the bacteria,incubated in TSBA(Trypticase soy broth agar),except Bacillus thuringiensis, whereas it is not so ideal,when the bacteria are incubated in BPA(beef peptone agar).A better result can be obtained when the conserved bacteria are activated twice or for 48 hours than for 24 hours.No difference is found in identification between bacteria sampled from quadrant 3 and quadrant 2. |
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| Keywords: | Sherlock MIS |
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