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| 肉苁蓉种子生活力快速测定方法研究 | |
| 引用本文： | 徐荣,陈君,周峰,于晶,刘同宁.肉苁蓉种子生活力快速测定方法研究[J].种子,2011,30(5). |
| 作者姓名： | 徐荣 陈君 周峰 于晶 刘同宁 |
| 作者单位： | 1. 中国医学科学院药用植物研究所濒危药材繁育国家重点实验室,北京,100193 2. 上海市农业科学院食用菌研究所,上海,201106 3. 宁夏永宁县本草苁蓉种植基地,银川,750100 |
| 基金项目： | 国家科技重大专项，国家自然科学基金项目，国家科技支撑计划项目 |
| 摘 要： | 采用单因素、多因素交互及正交试验设计等方法,对肉苁蓉种子生活力测定过程中的预处理方法四唑染色液浓度及pH值进行优化,初步建立了肉苁蓉种子生活力的快速测定技术,使测定程序简化、时间缩短,并确定38℃下去皮直接染色18 h的最适宜染色液pH值为6.4,TTC浓度为0.3%～1.0%. |
| 关 键 词： | 肉苁蓉 种子生活力 快速测定 |
Study on Rapid Determination Technique of Cistanche deserticola Seed Viability |
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| XU Rong,CHEN Jun,ZHOU Feng,YU Jing,LIU Tong-ning.Study on Rapid Determination Technique of Cistanche deserticola Seed Viability[J].Seed,2011,30(5). | |
| Authors: | XU Rong CHEN Jun ZHOU Feng YU Jing LIU Tong-ning |
| Institution: | XU Rong1,CHEN Jun1,ZHOU Feng2,YU Jing1,LIU Tong-ning3(1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences / National EngineeringLaboratory for Breeding of Endangered Medicinal Materials,Beijing 100094,China,2.Institute of Edible Fungi,Shanghai Academy of Agricultural Science,Shanghai 201106,3.Ningxia Yongning Plantation of Herba Cistanche,Yinchuan 750100,China) |
| Abstract: | Traditional single-factor experiment,multi-factors interacted experiment,and orthogonal experiment were applied to improve the pretreatment methods,concentration and pH value of 2,3,5-triphenyl tetrazolium chloride(TTC) solution in the seed viability determination process.A rapid determination technique of Cistanche deserticola seed viability was primarily established by TTC test,which progress was simplified and reaction time was cut short.The optimum pH value and TTC solution concentration was 6.4 and 0.3... |
| Keywords: | Cistanche deserticola seed viability rapid determination |
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