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| 缘盖牛肝菌纯培养菌种的RAPD和ITS分子标记鉴定 | |
| 引用本文： | 李海波,吴学谦,应国华,魏海龙,付立忠,吴庆其,许琳,柳青. 缘盖牛肝菌纯培养菌种的RAPD和ITS分子标记鉴定[J]. 食用菌学报, 2007, 14(1): 1-9 |
| 作者姓名： | 李海波 吴学谦 应国华 魏海龙 付立忠 吴庆其 许琳 柳青 |
| 作者单位： | 1. 浙江省林业科学研究院生物技术研究所,浙江杭州,310023 2. 浙江省林业科学研究院生物技术研究所,浙江杭州,310023;丽水市食用菌研究开发中心,浙江益圣菌物发展有限公司,浙江丽水,323000 3. 丽水市林业科学研究所,浙江丽水,323000 4. 丽水市食用菌研究开发中心,浙江益圣菌物发展有限公司,浙江丽水,323000 |
| 基金项目： | 浙江省科技攻关项目;浙江省自然科学基金;丽水市重点科技攻关项目 |
| 摘 要： | Boletus appendiculatus(Schoeff:Fr.)Secretanis an ectomycorrhizal fungus normallyassociated withPinus taiwanensisandPinusdensiflora,andis distributed inP.taiwanensisforests or in mixed forests consisting mainly ofP.taiwanensisat elevations between800to1300… |
| 关 键 词： | 缘盖牛肝菌 鉴定 分子标记 纯培养 |
| 修稿时间： | 2006-11-01 |
Authentication of Fungal Mycelium Isolated from Boletus appendiculatus Fruit Body Tissue Using RAPD and ITS Markers |
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| LI Haibo,WU Xueqian,YING Guohua,WEI Hailong,FU Lizhong,WU Qingqi,XU Lin,LIU Qing. Authentication of Fungal Mycelium Isolated from Boletus appendiculatus Fruit Body Tissue Using RAPD and ITS Markers[J]. Acta Edulis Fungi, 2007, 14(1): 1-9 | |
| Authors: | LI Haibo WU Xueqian YING Guohua WEI Hailong FU Lizhong WU Qingqi XU Lin LIU Qing |
| Abstract: | RAPD molecular markers and ITS sequence comparisons were used to establish the relationship between Boletus appendiculatus fruit bodies collected from Qingyuan, Zhejiang Province, and mycelium isolated from the fruit body tissue. RAPD analysis of extracted genomic DNA revealed that B. appendiculatus fruit bodies (fb) and the isolated fungal mycelium (mb) formed a single cluster with a Dice coefficient value of 0.92. Comparison of ITS sequences from these samples showed that base sequences and fragment lengths were identical, thereby confirming that the fungal mycelium isolated from B. appendiculatus fruit bodies was the vegetative form of the mushroom. Our data suggest that RAPD fingerprinting combined with ITS sequence comparisons constitute an effective and feasible method for identifying mycelium isolated from ectomycorrhizal fungi. |
| Keywords: | Boletus appendiculatus Identification Molecular marker Pure culture |
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