Tylosin detection in animal feed by liquid chromatography-tandem mass spectrometry with enzymatic hydrolysis of the tylosin urea adduct |
| |
Authors: | Van Poucke Christof Dumoulin Fréderic De Keyser Kirsten Elliott Chris Van Peteghem Carlos |
| |
Affiliation: | Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium. christof.vanpoucke@UGent.be |
| |
Abstract: | When the use of tylosin as a feed additive was forbidden by Council Regulation 2821/98, the necessity of a chemical confirmation method for the monitoring of the ban was created. Recently a method was developed for the detection of tylosin in animal feed by means of LC-MS/MS. During the validation high deviating values for the decision limit, detection capability, and repeatability for tylosin in cattle feed were observed, and the presence of urea and the formation of a tylosin urea adduct (TUA) were suggested as possible explanations. In this study two hydrolysis approaches for the TUA adduct were compared, namely, a chemical hydrolysis and an enzymatic hydrolysis with urease. The latter yielded a more complete hydrolysis of urea and was used for further validation. The recovery increased by approximately 15-25% depending on the amount of urea present in the feed (0.5-2%). The decision limit and detection capability were hardly influenced by the enzymatic hydrolysis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|