新疆紫草快繁技术研究

[目的]建立新疆紫草组培快繁技术体系.[方法]以新疆紫草无菌苗的茎尖为外植体,筛选适合茎尖萌发、增殖、生根的激素组合.[结果]茎尖最佳萌发培养基为MS+TDZ 1.0 mg/L,其萌发率为100;.芽增殖培养基为MS+TDZ 1.0 mg/L,芽增殖倍数为13.50倍,继代壮苗培养基为MS+NAA 0.3 mg/L +6-BA 0.5 mg/L,生根培养基为MS+NAA 0.1 mg/L,生根率为84;.降低6-BA浓度到0.05 mg/L可大幅降低玻璃化发生率.经过此培养基继代后,再在原生根配方中生根,生根率提高到95.32;.[结论]茎尖是新疆紫草快繁的最佳外植体.

Rapid Propagation of Arnebia euchroma(Royle.)

【Objective】The aim of the study was to establish rapid propagation system of Arnebia euchroma(Royle.) Johnst.【Method】The proper combination of growth regulator was screened by using the stem-tip of Arnebia euchroma(Royle) as a explant,proliferate and root-age.【Result】The results showed that the optimal germination medium was MS+TDZ 1.0 mg/L,whose germination rate was 100% and the bud multiplication medium was MS+TDZ 1.0 mg/L with 13.50 folds and successive healthy seedling medium was MS+NAA 0.3 mg/L+6-BA 0.5 mg/L,the best rooting medium was MS+NAA 0.1 mg/L,the rate of rooting was 84%.Where the consentration of 6-BA decreased by 0.05,the rate of vitrification could be largely decreased and when cultured in this medium,the rooting rate could increased to 95.32%.【Conclusion】Stem tip is the best explant of Arnebia euchroma(Royle.) Johnst rapid propagation