| 鳞球茎茎线虫实时荧光PCR检测技术的研究 |
| |
| 引用本文： | 王翀,葛建军,陈长发.鳞球茎茎线虫实时荧光PCR检测技术的研究[J].植物检疫,2005,19(1):11-14. |
| |
| 作者姓名： | 王翀 葛建军 陈长发 |
| |
| 作者单位： | 1. 中国检科院动植物检疫研究所,北京,100029
2. 青岛出入境检验检疫局 |
| |
| 摘 要： | 传统的线虫形态学鉴定存在经验性强和幼虫难以鉴定等不足.本文根据线虫ITS基因序列多态性,设计鳞球茎茎线虫(Ditylenchus dipsaci)特异的TaqMan探针,建立了实时荧光PCR检测方法.对茎线虫属的鉴定实验表明:只有鳞球茎茎线虫产生特异性荧光信号,同属的其他线虫均检测到荧光信号.整个检测过程只需30min到2h,能够满足口岸检验检疫快速通关的要求.
|
| 关 键 词： | TaqMan探针 实时荧光PCR 鳞球茎茎线虫 |
Detection of Ditylenchus dipsaci by real time PCR |
| |
| Wang Chong,Ge Jianjun.Detection of Ditylenchus dipsaci by real time PCR[J].Plant Quarantine,2005,19(1):11-14. |
| |
| Authors: | Wang Chong Ge Jianjun |
| |
| Abstract: | Stem bulb nematode(Ditylenchus dipsaci )is a dangerous and damage nematodes in the world,and is also in the A 2 li st of quarantine pests concerned to China.A real time PCR method was developed for detection of D.dipsaci.The species-specific probe for D.dipsaci was developed,and no fluorescent signal was detected fo r other nematode except D.dipsaci.The detection process only n eeds 30min or 2 hours,so the method is specific and little time consumption. |
| |
| Keywords: | Taq Man probe real-time PRC Ditylenchus dip saci |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
