高浓度臭氧对春小麦膜脂过氧化和抗氧化系统的影响

利用开顶式气室(OTC)研究了高浓度O3对春小麦叶片膜脂过氧化程度、活性氧含量、抗氧化系统的影响。结果表明,随着臭氧浓度的升高,不同生育时期小麦叶片的相对电导率、MDA含量显著上升(P<0.01);穗粒重明显下降(P<0.05);O2-.产生速率和H2O2含量显著增加(P<0.05)。SOD活性、CAT活性、POD活性随生育进程和O3浓度升高而显著下降。而对抗氧化物如类Car含量、ASA含量的影响不显著。臭氧浓度增加,促使小麦叶片膜的伤害,引发了膜脂过氧化作用,产生了过多的活性氧自由基,破坏了抗氧化系统功能,影响了叶片的正常生理代谢。

Effect of ozone on membrane lipid and antioxidation system of wheat leaves

To examine the effects of ozone on lipid peroxidation,and anti-oxidation system of the seedlings of wheat,field study was carried out by using open top chamber(OTCs).The results revealed that with ozone concentration rising,the relative electric conductivity and MDA content of the leaves increased.Compared to the CK treatment,ozone treatments caused a 20.9% decrease in grain weight of ear remarkably.The trend of changes of O^-₂ producing rates of wheat leaves, H₂O₂ content increased under ozone treatment.The SOD,CAT and POD activities declined in various stages of wheat growth and this change appeared remarkable.No significant change was detected about antioxidants such as the carotinoid content and ascorbate content.Accordingly,it can be seen that ozone intensify the damage to their membrane lipid,and impair the function of their antioxidation system,in such a way exerting influence on their regular physiological metabolism.