| 百合珠芽组培及脱毒研究 |
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| 引用本文： | 赵祥云,程廉.百合珠芽组培及脱毒研究[J].园艺学报,1993,20(3):284-288. |
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| 作者姓名： | 赵祥云 程廉 |
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| 作者单位： | 西北农业大学，西北农业大学，西北农业大学，西北农业大学，西北农业大学 陕西杨陵 712100北京农学院园林系，陕西杨陵 712100，陕西杨陵 712100，陕西杨陵 712100，陕西杨陵 712100 |
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| 摘 要： |
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| 关 键 词： | 百合 珠芽 脱毒 组织培养 |
Studies on Bulblet Culture and Devirus of Lilium sulphureum Baker |
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| Zhao Xiangyun,Cheng Lian,Xing Youmei,Xie Liping,and Jia Xuewen.Studies on Bulblet Culture and Devirus of Lilium sulphureum Baker[J].Acta Horticulturae Sinica,1993,20(3):284-288. |
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| Authors: | Zhao Xiangyun Cheng Lian Xing Youmei Xie Liping and Jia Xuewen |
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| Abstract: | The growing points in bulblets of Lilium sulphureum Baker with tobacco ringspot virus (TRSV) were used for devirus by means of tissue cultue.This experiment showed that the most suitable medium on which callus was induced from the growing points of the bulblets was MS+BA0.5+2,4-D0.25mg/ 1 and the inductivity was 92.8%.The medium suitable for bud differentiation was MS+BA1.5+NAA 0.1+KT0.lmg/ 1 and the rate of differentiation was 71.4%.l / 2MS+IBA0.25mg/ 1 was fit for root growing and the multiplication rate was 100% . It took 120~ 150 days to develop plantlets from the explants of Lilium sulphureum Baker. These plantlets were used for devirus identification by six indicator plants. The results showed that the TRSV was successfully eliminated in the plantlets induced from the growing points of the bulblets 0.3 ~ 0.8mm long. |
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| Keywords: | Lilium sulphureum Bulblets Tissue culture Devirus Tobacco ringspot virus (TRSV) |
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