| A蛋白斑点免疫金染色和双抗体夹心酶联检测齿兰环斑病毒的比较 |
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| 引用本文： | 魏梅生,黄冲.A蛋白斑点免疫金染色和双抗体夹心酶联检测齿兰环斑病毒的比较[J].植物检疫,2000,14(6):344-346. |
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| 作者姓名： | 魏梅生 黄冲 |
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| 作者单位： | 1. 国家出入境检验检疫局动植物检疫实验所,北京,100029
2. 西北农林科技大学植保系 |
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| 摘 要： | A蛋白斑点免疫金染色检测齿兰环斑病毒提纯病毒的灵敏度为1.56 ng/μl,检测病汁液的稀释倍数为640倍.同时采用碱性磷酸酯酶标记抗体IgG.IgG包被酶联板的浓度为1 μg/ml,酶标抗体以1 /1000稀释使用.DAS-ELISA技术检测ORSV提纯病毒的灵敏度为0.048 ng/μl,检测病汁液的稀释倍数为20480倍.
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| 关 键 词： | A蛋白斑点免疫金染色 齿兰环斑病毒 酶联检测 |
Detection of Odontoglossum ringspot virus by Protein-A-gold complexes and DAS-ELISA |
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| Wei Meisheng,Huang Chong.Detection of Odontoglossum ringspot virus by Protein-A-gold complexes and DAS-ELISA[J].Plant Quarantine,2000,14(6):344-346. |
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| Authors: | Wei Meisheng Huang Chong |
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| Abstract: | A method for detecting Odontoglossum ringspot virus on nitrocellulose by protein A gold complexes has been developed.It involved specific detection of virus by polyclonal antiserum followed staining with protein A bound to particles of colloidal gold.the amount of virus detectable was 1.56ng.The sensitivity was then comparable to DAS ELISA using alkaline phosphatase labeled antibody. |
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| Keywords: | Protein A gold complex Odontoglossum ringspot virus DAS ELISA |
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