一株鸭致病性大肠杆菌的分离鉴定及耐药分析

对江西省樟树市某鸭养殖场送检的病死鸭进行病理剖检,通过细菌分离、生化鉴定得到1株致病性大肠杆菌。药敏试验结果表明,该菌株对头孢拉定、头孢唑啉、头孢曲松和阿莫西林等β-内酰胺类抗生素产生了严重的耐药。通过质粒提取和接合转移试验对分离菌株进行耐药性分析;提取得到一10 kb左右的质粒;质粒接合转移试验表明,成功将该质粒转化入感受态E.coli JM109,并能使E.coli JM109获得对β-内酰胺类抗生素耐药且与分离菌株的耐药谱一致。推测该菌株产β-内酰胺酶的基因存在于质粒,并且该耐药基因能随质粒的转移转化入敏感菌E.coli JM109。

Isolation,Identification and Drug Resistance Analysis of One Strain of Escherichia coli from Ducks

One pathogenic Escherichia coli was isolated and identified from dead ducks from Zhangshu city of Jiangxi province.Drug sensitivity test indicated that the isolate had a strong resistance with cefradine,cefazolin,ceftriaxone and amoxicillin which were belong to β-lactams.Through extracting plasmid of the isolated and transfering into drug-sensitive E.coli JM109 to analyze the antibiotic resistance,the result showed that there was a 10 kb plasmid and successeful transfered to E.coli JM109,which maked the sensitive E.coli JM109 had the same drug resistance as the isolate.It′s speculated that the resistance gene existed on the plasmid of pathogenic Escherichia coli and the resistance gene could be transferred with plasmid.