大蜡螟作寄主繁殖古巴蝇接种试验

为利用大蜡螟大规模繁殖及应用古巴蝇,解剖观察了古巴蝇雌虫体内幼虫量,并进行大蜡螟幼虫接种不同古巴蝇幼虫量以及不同生长期大蜡螟幼虫做寄主对古巴蝇蛹数、大蜡螟存活数和死亡数的影响试验。结果表明,古巴蝇雌虫体内平均幼虫量达170多条;大蜡螟接种不同古巴蝇幼虫量后,所得的古巴蝇蛹数随着接种量的增大呈上升趋势,但35~38条与45~48条蛆的接种量所得古巴蝇蛹数差异不显著;不同生长期大蜡螟接种古巴蝇后,古巴蝇收蛹数随着寄主大蜡螟生长期延长而增加,生长40 d和50 d的大蜡螟做寄主时,古巴蝇收蛹数、大蜡螟存活数和死亡数差异不显著。考虑到寄主饲料成本和羽化时间等因素,35~38条接蛆量和40 d生长期的大蜡螟做寄主为最佳选择。

Trial of Lixophaga diatraeae Townsend propagation inoculated on host Galleria mellonella

The present study has been conducted to observe the larva number in female adult of Lixophaga diatraeae in order to make mass propagation and utilization of Lixophaga diatraeae using host Galleria mellonella,and to find out the effects of number of Lixophaga diatraeae larva inoculation and the host Galleria mellonella during different growth period on number of Lixophaga diatraeae pupa and survival and death number of Galleria mellonella.The results revealed that number of larva in each female Lixophaga diatraeae was over 170 on an average.The number of Lixophaga diatraeae pupa was added with increase of inoculation lava number,which didn’t have significant difference between 35-38 and 45-48 inoculation number.The number of Lixophaga diatraeae pupa was added with increase in growth period of host Galleria mellonel,and the number of Lixophaga diatraeae pupa,survival and death number of Galleria mellonella didn’t have significant difference between 40 and 50 days growth period.Considering feed cost and emergency time of host,35-38 inoculation number of Lixophaga diatraeae larva and 40 days growing period of host Galleria mellonella have been found to be the best.