Improvement in a simple method for isolating white spot syndrome virus (WSSV) from the crayfish Procambarus clarkii |
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| Affiliation: | 1. Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou-310029, PR China;2. Suzhou University, Suzhou-215006, PR China;1. State Key Laboratory for Biocontrol / School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China;2. School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China;3. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, PR China;1. School of Basic Medical Sciences, Binzhou Medical University, Yantai, 264003, Shandong, China;2. State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, Hainan, China;3. Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, 264003, Shandong, China;1. Centre for Biotechnology, Anna University, Chennai, India;2. National Center for Aquatic Animal Health, Cochin University of Science and Technology, Cochin, India;3. Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø, Norway |
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| Abstract: | A very simple and efficient protocol for purification of intact White spot syndrome virus (WSSV) viral particles from infected tissues was developed using crayfish, Procambarus clarkii, as a proliferation system. No density gradient centrifugation, ultracentrifugation or protease inhibitors were needed and intact WSSV virions could be purified using a 30% sucrose solution combined with a few steps of differential centrifugation. Virus DNA quantitative analysis and infection trial suggested that the purity of the virions was sufficiently high for subsequent experimental work. |
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