沙田柚柚皮提取物的抗菌性能研究

[目的]为柚皮的综合利用提供参考依据。[方法]用乙醇提取沙田柚柚皮中的有效成分,滤纸片扩散法测定柚皮提取物的抑菌活性,研究温度对柚皮提取物抑菌活性的影响,比较柚皮中的主要成分黄酮和香精油的抑菌作用。[结果]柚皮提取物对枯草芽孢杆菌具有较强的抑制作用,与100μg/ml的青霉素效果相当,对大肠杆菌、金黄色葡萄球菌及酵母菌的抑制作用相对较弱,对米曲霉和黑曲霉没有明显的抑菌效果,施用7 h时抑菌圈较大,10 h后抑菌圈逐渐变小。温度对柚皮提取物的抑菌活性有明显影响。柚皮中的黄酮和香精油都具有抑菌作用,且香精油的抑菌活性低于黄酮。[结论]柚皮提取物具有良好的抑菌作用。

study on the Antibacterial Activity of the Extract from Peel of Pomelo

[Objective] The study aimed to provided the reference for comprehensive utilization of shaddock peel.[Method] The ethanol was used to extract the effective components of pomelo peel,and the filter paper method was used to detect the antibacterial activity of pomelo peel extract.The research studied the influence of the temperature on the antibacterial activity of pomelo peel extract.The inhibitory effects of two main components from pomelo peel,flavone and essential oil,were compared.[Result] The pomelo peel extract exhibited the strong inhibitory effect on Bacillus subtilis,with inhibitory effect being equivalent to that of 100 μg/ml penicillin,and had slightly inhibitory effects on Eseherichia coli,Staphylococcus aureus,and Yeast.There was no significant inhibitory effect on Aspergillus oryzae and Aspergillus niger.The inhibition zone was largest when applying the extract for 7 h and was decreased after 10 h.The inhibitory effect of pomelo peel extract was obviously affected by temperature.Flavone and essential oil from pomelo peel all had inhibitory effects,but the latter was lower than the former.[Conclusion] The pomelo peel extract had better inhibitory effects.