| 超声波辅助对农杆菌介导“美人指”葡萄遗传转化中GUS瞬时表达的影响 |
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| 引用本文： | 周蓓蓓,陈月红,章镇,陶建敏.超声波辅助对农杆菌介导“美人指”葡萄遗传转化中GUS瞬时表达的影响[J].江西农业学报,2010,22(4):6-8. |
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| 作者姓名： | 周蓓蓓 陈月红 章镇 陶建敏 |
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| 作者单位： | 1. 南京农业大学,园艺学院,江苏,南京,210095;江苏省农业科学院,园艺研究所,江苏,南京,210014
2. 南京农业大学,园艺学院,江苏,南京,210095 |
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| 基金项目： | 江苏省科技厅项目(BG2007309) |
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| 摘 要： | 通过超声波辅助农杆菌介导法,优化"美人指"葡萄遗传转化体系。利用GUS瞬时表达的方法研究了不同功率、时间条件下的超声波处理,外植体类型以及AS浓度对"美人指"葡萄遗传转化效率的影响。结果表明:外植体茎段在含75mg/L AS农杆菌悬浮液中侵染4 min,并在此期间,用功率为90 W的超声波处理14 s,获得最佳GUS瞬时表达率42.8%。
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| 关 键 词： | 葡萄 超声波辅助农杆菌介导 遗传转化 |
Effect of Sonication-assisted Agrobacterium-mediated Genetic Transformation on Instantaneous Expression of GUS in Grape Variety "Meirenzhi" |
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| ZHOU Bei-bei,CHEN Yue-hong,ZHANG Zhen,TAO Jian-min.Effect of Sonication-assisted Agrobacterium-mediated Genetic Transformation on Instantaneous Expression of GUS in Grape Variety "Meirenzhi"[J].Acta Agriculturae Jiangxi,2010,22(4):6-8. |
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| Authors: | ZHOU Bei-bei CHEN Yue-hong ZHANG Zhen TAO Jian-min |
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| Institution: | 1.College of Horticulture/a>;Nanjing Agricultural University/a>;Nanjing 210095/a>;China/a>;2.Institute of Horticulture/a>;Jiangsu Academy of Agricultural Sciences/a>;Nanjing 210014/a>;China |
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| Abstract: | In order to optimize the sonication-assisted Agrobacterium-mediated genetic transformation system of grape variety Meirenzhi,the effects of sonication treatment with different powers of ultrasonic for different time,different explant types and AS concentrations on the transformation efficiency were studied through the method of GUS instantaneous expression.The results showed that infecting explant stem segment with 75 mg/L AS suspension for 4 min,and in this period conducting sonication treatment with 90 W ... |
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| Keywords: | Grape Sonication-assisted Agrobacterium-mediated Genetic transformation |
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