| 酶联免疫吸附法(ELISA)检测豆类种传病毒的研究 |
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| 引用本文： | 张成良,张作芳,胡伟贞,宋淑敏,赵瑞生,马德芳,高乔婉. 酶联免疫吸附法(ELISA)检测豆类种传病毒的研究[J]. 植物保护学报, 1982, 9(1): 9-14 |
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| 作者姓名： | 张成良 张作芳 胡伟贞 宋淑敏 赵瑞生 马德芳 高乔婉 |
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| 作者单位： | 农业部植物检疫实验所，农业部植物检疫实验所，农业部植物检疫实验所，农业部植物检疫实验所，农业部植物检疫实验所，中国科学院微生物所，华南农学院 |
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| 摘 要： | 本文介绍使用微量板酶联免疫吸附法(ELISA)检测TMV(杆状)、SMV(线状)、CMV(球状)、AMV(杆菌状多粒体)四种不同形态、不同分类组群的病毒。此法非常灵敏,能够检测病毒提纯液的最低浓度为7.6～10亳微克/毫升,也能检出稀释1563～10240倍病叶粗澄清液中的病毒,灵敏度比琼脂双扩散法高1000倍以上。 ELISA可以直接检出单粒种子中的病毒,而且可以分别检出单粒种子不同部位如大豆的种皮、种胚、胚乳及胚根中的病毒存在。该法适用于检测大量标样,对植物检疫来说,这是一个很好的方法。
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STUDIES ON THE DETECTION OF SEED-BORNE VIRUS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) |
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| Zhang Chengliang,Zhang Zuofang,Hu Weizhen,Song Shumin,Zhao Ruisheng,Ma Defang and Gao Qiaowan. STUDIES ON THE DETECTION OF SEED-BORNE VIRUS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)[J]. Acta Phytophylacica Sinica, 1982, 9(1): 9-14 |
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| Authors: | Zhang Chengliang Zhang Zuofang Hu Weizhen Song Shumin Zhao Ruisheng Ma Defang Gao Qiaowan |
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| Affiliation: | Plant Quarantine Institute, Ministry of Agriculture,Plant Quarantine Institute, Ministry of Agriculture,Plant Quarantine Institute, Ministry of Agriculture,Plant Quarantine Institute, Ministry of Agriculture,Plant Quarantine Institute, Ministry of Agriculture,Institute of Microbiology, Academia Sinica and South-China Agricultural College |
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| Abstract: | The microplate enzyme-linked immunosorbent assay (ELISA) of determining the viruses of four different shapes (rigid red,long flexible thread,isometric and bacillus-like) and of different groupes was described.With a high sensitivity,it can determine the clarified virus of minimum cencentration at 7.6-10ng/ml and also determine the virus of the diseased leaf sap being diluted to 1563-10240 times.The sensitivityof this method is 1000 times higher than that of the agardouble diffu-sion test.The virus in a single seed and in various parts of a single seedchsnas skin germ,endosperm,and radicle of soybean seed could be detectedby ELISA.It can be used on large-scale samples,and is a very goodmethod applied to plant quarantine. |
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