家蚕表皮多酚氧化酶的分离纯化及酶学性质研究

[目的]分离纯化家蚕多酚氧化酶，并对其性质进行分析研究。[方法]采用硫酸铵分级沉淀、凝胶过滤、蛋白质电泳等方法。[结果]多酚氧化酶分子量约为81kD；最适温度为30℃；最适pn值为7．0。低浓度金属离子Ag^＋、Ca^2＋、Mn^2＋、Zn^2＋对多酚氧化酶有一定的激活作用，而高浓度有抑制作用。[结论]金属离子对家蚕多酚氧化酶的影响很大，值得更深入的研究。

Study on the Separation,Purification and Enzymatic Properties of Polyphenoloxidase from Epidermis of Bombyx mori

[Objective] The research aimed to separate and purify polyphenoloxidase from Bombyx mori and study its properties.[Method] The methods of ammonium sulfate precipitation,gel filtration,protein electrophoresis and so on were used.[Result] The molecular weight of polyphenoloxidase was about 81 kD.The optimum temperature was 30 ℃ and the optimum pH was 7.0.The metal ions of Ag+,Cu2+,Mn2+ and Zn2+ at low concentration had certain activation on polyphenoloxidase and that at high concentration had inhibition.[Conclusion] The influences of heavy metal ions on polyphenoloxidase from Bombyx mori were greater,to be worth further study.