| 种衣剂对大豆根际固氮菌多样性的影响 |
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| 引用本文： | 韩雪,孙龙,仕相林,尹晓玉,文景芝.种衣剂对大豆根际固氮菌多样性的影响[J].中国油料作物学报,2010,32(1):132-135. |
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| 作者姓名： | 韩雪 孙龙 仕相林 尹晓玉 文景芝 |
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| 作者单位： | 东北农业大学农学院，哈尔滨 150030
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| 基金项目： | 黑龙江省"十一五"重点项目，黑龙江省博士后启动金 |
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| 摘 要： | 利用变性梯度凝胶电泳(DGGE)技术研究宁南霉素、甲霜灵、多克福和咯菌腈4种种衣剂对大豆根际固氮菌多样性的影响。运用nifH基因的特异引物对,将大豆根际土壤总DNA进行PCR扩增后,通过DGGE技术对PCR产物进行分析。结果表明,种衣剂对固氮菌产生了不同程度的抑制作用。对大豆根际固氮菌抑制作用由强到弱的种衣剂品种依次是咯菌腈、甲霜灵、多克福和宁南霉素;种衣剂浓度对大豆根际固氮菌多样性的抑制程度不同,但没有明显规律。
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| 关 键 词： | 固氮菌 nif H基因 多样性 PCR-DGGE |
Impact of seed coatings on diversity of soybean rhizospheric azotobacter |
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| HAN Xue,SUN Long,SHI Xiang-lin,YIN Xiao-yu,WEN Jing-zhi.Impact of seed coatings on diversity of soybean rhizospheric azotobacter[J].Chinese Journal of Oil Crop Sciences,2010,32(1):132-135. |
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| Authors: | HAN Xue SUN Long SHI Xiang-lin YIN Xiao-yu WEN Jing-zhi |
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| Institution: | HAN Xue,SUN Long,SHI Xiang-lin,YIN Xiao-yu,WEN Jing-zhi(Department of Plant Protection,Agricultural College,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The impact of different seed coatings on diversity of soybean rhizospheric azotobacter was analyzed by detection of nif H gene diversity using denaturing gradient gel electrophoresis (DGGE). The results indicated that different kinds of seed coatings were suppressive to soybean rhizospheric azotobacter. The suppressive effect in a decreasing order was Fludioxonil,Metalaxyl,Carbofuran-Carbendazim-Thiram and Ninanmycin. The suppressive effects varied with the concentration of seed coatings without clear regul... |
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| Keywords: | PCR-DGGE Azotobacter nif H gene Diversity PCR-DGGE |
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