| 辐照对结缕草农杆菌介导法遗传转化的影响 |
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| 引用本文： | 张磊,胡繁荣,张琳琳,王雪艳,马传喜,吴殿星.辐照对结缕草农杆菌介导法遗传转化的影响[J].核农学报,2004,18(6):435-437,442. |
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| 作者姓名： | 张磊 胡繁荣 张琳琳 王雪艳 马传喜 吴殿星 |
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| 作者单位： | 浙江大学原子核农业科学研究所,浙江,杭州,310029;安徽农业大学农学系,安徽,合肥,230036;浙江大学原子核农业科学研究所,浙江,杭州,310029;安徽农业大学农学系,安徽,合肥,230036 |
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| 基金项目： | 国家自然科学基金 ( 3 0 3 70 14 7)，浙江省重大项目 ( 0 2 110 2 5 3 0 ) |
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| 摘 要： | 以结缕草成熟种子诱导产生的胚性愈伤组织为材料 ,研究了钴 60伽玛射线辐照对农杆菌介导法遗传转化的影响。结果表明 ,在辐照剂量为 1～ 4Gy时 ,GUS瞬间表达率随剂量的增加逐渐提高 ,但在辐射剂量高于 4Gy时 ,GUS瞬间表达率随剂量的增加而下降。综合考虑辐照后愈伤组织的存活率和GUS瞬间表达率 ,初步认为 2Gy为最佳辐照剂量。进一步观察表明 ,辐照处理后以继代培养 36h最适合农杆菌介导感染。
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| 关 键 词： | 结缕草 伽玛辐照 GUS瞬间表达 |
| 文章编号： | 1000-8551(2004)06-435-03 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
EFFECT OF GAMMA IRRADIATION ON Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF JAPANESE LAWNGRASS(Zoysia japonica Steud.) |
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| ZHANG Lei , HU Fan-rong ZHANG Lin-lin WANG Xue-yan MA Chuan-xi WU Dian-xing.EFFECT OF GAMMA IRRADIATION ON Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF JAPANESE LAWNGRASS(Zoysia japonica Steud.)[J].Acta Agriculturae Nucleatae Sinica,2004,18(6):435-437,442. |
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| Authors: | ZHANG Lei HU Fan-rong ZHANG Lin-lin WANG Xue-yan MA Chuan-xi WU Dian-xing |
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| Institution: | ZHANG Lei 1,2 HU Fan-rong1 ZHANG Lin-lin1 WANG Xue-yan1 MA Chuan-xi2 WU Dian-xing 1* |
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| Abstract: | The effects of gamma irradiation on Agrobacterium-mediated genetic transformation of were investigated in the current paper, using embryonic calli derived from the mature seeds of Japanese lawngrass(Zoysia japonica Steud.). The result indicated that the GUS transient expression rates were enhanced with the increasing doses when treated by doses lower than 4Gy, however it would be decreased when treated by doses higher than 4Gy. Based on the survival rate and GUS transient expression rate, 2Gy is the optimal dose for Agrobacterium-mediated genetic transformation. Further observation found that 36 hours reculture after gamma irradiation is the most appropriate for Agrobacterium infection. |
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| Keywords: | Japanese lawngrass gamma irradiation GUS transient expression |
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