鸭源新城疫病毒的分离与鉴定

[目的]分离并鉴定鸭源新城疫病毒。[方法]从安徽地区鸭病料中分离新城疫病毒,测定其鸡胚半数感染量(EID_(50))、鸡胚平均致死时间(MDT)和1日龄雏鸡脑内接种致病指数(ICPI)。[结果]2株新城疫病毒均为强毒。通过RT-PCR技术对这2株鸭新城疫病毒的F基因进行序列测定与分析,结果发现F基因裂解位点为~(112)RRQKRF~(117),符合NDV强毒株裂解位点氨基酸序列。F基因遗传进化树分析发现,AH1株属于基因Ⅶ型,而AH2株属于基因Ⅵ型,来源于鸽源病毒。[结论]水禽新城疫病毒(NDV)的感染来源复杂,应避免将不同种类的家禽混合饲养。

Separation and Identification of Duck-origin Newcastle Disease Virus

[Objective] To separate and identify newcastle disease virus (NDV) from duck.[Method] NDV were separated from diseased duck samples from Anhui Province and their EID50,MDT and ICPI were determined.[Result] Two strains of NDV were virulent.F gene of two strains of NDV were sequenced by RT-PCR,and the results showed that the cleavage site of F gene of the viruses was 112RRQKRF117,which was accorded with the amino acid sequences of cleavage site of highly-pathogenic strain of NDV.The genetic evolutionary tree analysis of F gene showed that AH1 belonged to genotype Ⅶ,but AH2 belonged to genotype Ⅵ,which was from pigeon sources.[Conclusion] The infection sources of NDV from water birds were complex,so the mixed breeding of different varieties of fowls should be avoided.