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水培条件下pH对大蒜生长趋势的影响
引用本文:朱惠敏,梁丽影.水培条件下pH对大蒜生长趋势的影响[J].安徽农业科学,2017,45(26).
作者姓名:朱惠敏  梁丽影
作者单位:肇庆学院生命科学学院,广东肇庆,526000;肇庆学院生命科学学院,广东肇庆,526000
摘    要:目的]研究水培大蒜在不同pH条件下的生长发育及SOD活性变化。方法]土壤溶液的pH对植物的生长发育有重要的影响,试验以不同梯度的pH水溶液水培大蒜,在不同生长阶段对其SOD活性进行测定。试验采用丙酮沉淀法分离纯化SOD沉淀,用NBT光还原法测定其活性。结果]水培大蒜在pH为6.0时长势最好。在pH 4.55.5的生长环境下,大蒜的SOD活性相对稳定,但伴随着逐渐生长,SOD活性呈现上升趋势。酸性环境下,SOD活性相对较高。结论]该研究可为大蒜的水培提供指导。

关 键 词:水培大蒜  pH  SOD活性  NBT光还原法

Effects of pH on Growth Trend of Hydroponic Garlic
ZHU Hui-min,LIANG Li-ying.Effects of pH on Growth Trend of Hydroponic Garlic[J].Journal of Anhui Agricultural Sciences,2017,45(26).
Authors:ZHU Hui-min  LIANG Li-ying
Abstract: Objective] Growth and development and SOD activity changes of hydroponic garlic were studied under different pH. Method] Hy-droponics was adopted to study the effect of different gradient of pH aqueous solution on growth and development of plants. The SOD activity was measured at different growth stages. The SOD precipitation was separated and purified by using acetone precipitation, the activity was de-termined by NBT photo reduction method. Result] The best growth condition of hydroponic garlic was observed when the pH was 6. 0. Under the growth environment of pH 4. 5-5. 5, the SOD activity of garlic was relatively stable. With the gradual growth of germination, the SOD ac-tivity had rising tendency. SOD activity of garlic was relatively high in acid environment. Conclusion] The research can give guidance for hy-drophonic culturing garlic.
Keywords:Hydroponic garlic  pH  SOD activity  NBT photo reduction method
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