| 不洁贮藏对葡萄果肉衰老相关基因表达的影响 |
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| 引用本文： | 杜春望,陈璐璐,钱荣荣,葛腊梅,赵宇飞,王薇,蔡小宁,陈守江. 不洁贮藏对葡萄果肉衰老相关基因表达的影响[J]. 安徽农业科学, 2017, 45(16). DOI: 10.3969/j.issn.0517-6611.2017.16.039 |
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| 作者姓名： | 杜春望 陈璐璐 钱荣荣 葛腊梅 赵宇飞 王薇 蔡小宁 陈守江 |
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| 作者单位： | 南京晓庄学院食品科学学院,江苏南京,211171;南京晓庄学院食品科学学院,江苏南京,211171;南京晓庄学院食品科学学院,江苏南京,211171;南京晓庄学院食品科学学院,江苏南京,211171;南京晓庄学院食品科学学院,江苏南京,211171;南京晓庄学院食品科学学院,江苏南京,211171;南京晓庄学院食品科学学院,江苏南京,211171;南京晓庄学院食品科学学院,江苏南京,211171 |
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| 基金项目： | 江苏省大学生实践创新训练计划项目，南京晓庄学院重点项目，国家自然科学基金项目 |
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| 摘 要： | [目的]研究不洁贮藏对葡萄果肉衰老相关基因表达的影响。[方法]以巨峰葡萄果实为材料,应用实时荧光定量PCR技术对不同处理的相对表达量进行测定。[结果]ACC氧化酶基因、ACC合成酶基因、苯丙氨酸解氨酶基因表达量明显上调;脱落酸调控基因表达量明显下调。[结论]该试验为从基因水平上研究葡萄保鲜机理奠定了基础。
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| 关 键 词： | 葡萄 果肉 衰老相关基因 基因表达 |
Effect of Unclean Storage on Senescence-related Gene Expression in Grape Pulp |
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| DU Chun-wang,CHEN Lu-lu,QIAN Rong-rong,GE La-mei,ZHAO Yu-fei,WANG Wei,CAI Xiao-ning,CHEN Shou-jiang. Effect of Unclean Storage on Senescence-related Gene Expression in Grape Pulp[J]. Journal of Anhui Agricultural Sciences, 2017, 45(16). DOI: 10.3969/j.issn.0517-6611.2017.16.039 |
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| Authors: | DU Chun-wang CHEN Lu-lu QIAN Rong-rong GE La-mei ZHAO Yu-fei WANG Wei CAI Xiao-ning CHEN Shou-jiang |
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| Abstract: | [Objective] To study the effect of unclean storage on senescence-related gene expression in grape pulp.[Method] The relative expression of different treated genes was determined by real-time fluorescence quantitative PCR with Kyoho grape fruit as material.[Result] The expression of ACC oxidase gene,ACC synthase gene and phenylalanine ammonia lyase gene were significantly up-regulated.The expression of abscisic acid-regulated gene was down-regulated.[Conclusion] The experiment laid the foundation for the study of grape preservation mechanism from the genetic level. |
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| Keywords: | Grape Pulp Senescence-related gene Gene expression |
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