| 暗纹东方鲀微卫星筛选和群体遗传多样性分析 |
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| 引用本文： | 程长洪,张敏莹,刘凯,徐东坡,段金荣,周彦锋,施炜纲. 暗纹东方鲀微卫星筛选和群体遗传多样性分析[J]. 江西农业大学学报, 2011, 0(5) |
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| 作者姓名： | 程长洪 张敏莹 刘凯 徐东坡 段金荣 周彦锋 施炜纲 |
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| 作者单位： | 南京农业大学无锡渔业学院;中国水产科学研究院内陆渔业生态环境和资源重点开放实验室淡水渔业研究中心; |
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| 基金项目： | 公益性行业(农业)科研专项(200903048-03); 中央级公益性科研院所基本科研业务费专项资金资助项目(2011JBFA05,2011JBFA06) |
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| 摘 要： | 为了解长江野生暗纹东方鲀群体遗传多样性现状,采用已发表的16对红鳍东方鲀微卫星引物对暗纹东方鲀基因组DNA进行PCR扩增,结果有10对微卫星引物(占总数62.5%)能扩增出特异性条带。利用这10对微卫星引物对采自长江常熟江段30尾暗纹东方鲀群体进行扩增分析,共获得51个等位基因,每个位点等位基因数在2到10之间,平均为5.1。10个位点平均观察杂合度(Ho)为0.820 0,平均期望杂合度(He)为0.692 8,平均多态信息含量(PIC)为0.630 0。8个位点表现为高度多态(PIC>0.5),2个位点表现为中度多态(0.25
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| 关 键 词： | 红鳍东方鲀 暗纹东方鲀 微卫星引物 遗传多样性 |
Isolation of Polymorphic Microsatellite Loci and Genetic Diversity Analysis of Takifugu obscurus |
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| CHENG Chang-hong,,ZHANG Min-ying,LIU Kai,XU Dong-po,DUAN Jing-rong,ZHOU Yan-feng,SHI Wei-gang. Isolation of Polymorphic Microsatellite Loci and Genetic Diversity Analysis of Takifugu obscurus[J]. Acta Agriculturae Universitis Jiangxiensis, 2011, 0(5) |
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| Authors: | CHENG Chang-hong ZHANG Min-ying LIU Kai XU Dong-po DUAN Jing-rong ZHOU Yan-feng SHI Wei-gang |
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| Affiliation: | CHENG Chang-hong1,2,ZHANG Min-ying2,LIU Kai2,XU Dong-po2,DUAN Jing-rong2,ZHOU Yan-feng2,SHI Wei-gang2*(1.Wuxi Fisheriers College of Nanjing Agricultural University,Wuxi 214081,China,2.Key Laboratory of Ecological Environment and Resources of Inland Fisheries,Freshwater Fisheries Research Center,Chinese Academy of Fisheries Science,China) |
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| Abstract: | To investigate the genetic diversity of Takifugu obscurus in Yangtze River,16 microsatellite loci of Takifugu rubripes were analyzed for Takifugu obscurus.It was found that 6 loci failed to be amplified and 10 loci(about 62.5%) were amplified with specific products successfully and a total of 51 alleles were detected in the 10 polymorphic microsatellite loci.The allele numbers for each primer ranged from 2 to 10 and a mean of 5.1 alleles were found for each locus.The average observed heterozygosity(Ho),aver... |
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| Keywords: | Takifugu rubripes Takifugu obscurus microsatellite primer genetic diversity |
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