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柑橘病害生防放线菌的分离筛选及抑菌作用研究
引用本文:刘冰,黎园,丁莎,宋水林. 柑橘病害生防放线菌的分离筛选及抑菌作用研究[J]. 江西农业大学学报, 2011, 0(5)
作者姓名:刘冰  黎园  丁莎  宋水林
作者单位:江西农业大学农学院;
基金项目:江西省自然科学基金(2010GQN0056); 江西省教育厅青年科学基金项目(GJJ11085); 江西农业大学博士启动基金和江西农业大学校科技基金
摘    要:通过稀释法对从赣南柑橘园采集的土样进行微生物分离,获得细菌、真菌和放线菌共52株,其中真菌3株、细菌27株、放线菌22株。进一步通过皿内实验筛选到1株对柑橘炭疽病菌和溃疡病菌有较强抑制作用、且效果稳定的放线菌株ML27,对其进行抑菌活性测定和抑菌机理初探。结果表明,该菌株对炭疽病菌的抑制率为72.15%,对溃疡病菌的抑菌圈为2.05 cm;其抑菌机理主要是对真菌的细胞壁产生影响,对细菌的菌体具有溶解作用。

关 键 词:放线菌  柑橘炭疽病菌  柑橘溃疡病菌  拮抗作用  

Isolation and Inhibitory Effect of Actinomyce Strain against Orange Diseases
LIU Bing,LI Yuan,DING Sha,SONG Shui-lin. Isolation and Inhibitory Effect of Actinomyce Strain against Orange Diseases[J]. Acta Agriculturae Universitis Jiangxiensis, 2011, 0(5)
Authors:LIU Bing  LI Yuan  DING Sha  SONG Shui-lin
Affiliation:LIU Bing,LI Yuan,DING Sha,SONG Shui-lin(College of Agronomy,JiangXi Agricultural University,Nanchang 330045,China)
Abstract:The microorganisms were isolated from soil from orangery in Southern Jiangxi Province by dilution and 52 strains including 3 fungus,27 bacteria and 22 actinomyces were obtained.An actinomyce strain ML27 exhibited high inhibitory effect on the growth of Colletotrichum gloeosporioides and Xanthomonas axonopodis pv.citri in vitro,the inhibitory effect and mechanisms of which were investigated primarily.The results indicated that strain ML27 inhibited the growth of Colletotrichum gloeosporioides by 72.15% and t...
Keywords:actinomyces  Colletotrichum gloeosporioides  Xanthomonas axonopodis pv.citri  antagonistic action  
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