| 湖北海棠MhPR10多基因家族成员的特征分析 |
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| 引用本文： | 张计育,章镇,乔玉山,宣继萍,郭忠仁.湖北海棠MhPR10多基因家族成员的特征分析[J].江西农业学报,2011(11):1-4,16. |
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| 作者姓名： | 张计育 章镇 乔玉山 宣继萍 郭忠仁 |
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| 作者单位： | 江苏省中国科学院植物研究所;南京农业大学园艺学院; |
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| 基金项目： | 江苏省科技支撑计划项目 |
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| 摘 要： | 以水杨酸处理过的湖北海棠全长cDNA文库为模板,利用PCR技术对其PR10基因家族进行扩增,并利用生物信息学的方法进行序列和表达特性分析。实验结果表明:分离了16个PR10基因序列,序列编码区长度均为477 bp,编码159个氨基酸,分子量范围为17.53～17.85 kDa,等电点为5.06～6.40。根据序列的相似性和进化分析,该16个基因分为6个亚类。利用SWISS-MODEL对其6个亚类蛋白进行三维立体结构预测6,个亚类蛋白中均含有2个α螺旋和7个β折叠。表达预测结果表明:湖北海棠MhPR10基因家族可能在各器官中组成型表达,同时可能受水杨酸、病原菌和植食昆虫诱导表达。湖北海棠MhPR10基因家族成员可能参与植物的生长发育,在抵御生物胁迫和非生物胁迫的过程中起着非常重要的作用。
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| 关 键 词： | 湖北海棠 MhPR10基因家族 特征 |
Characteristic Analysis of MhPR10 Multigene Family in Hubei Provincial Malus hupehensis |
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| ZHANG Ji-yu,ZHANG Zhen,QIAO Yu-shan,XUAN Ji-ping,GUO Zhong-ren.Characteristic Analysis of MhPR10 Multigene Family in Hubei Provincial Malus hupehensis[J].Acta Agriculturae Jiangxi,2011(11):1-4,16. |
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| Authors: | ZHANG Ji-yu ZHANG Zhen QIAO Yu-shan XUAN Ji-ping GUO Zhong-ren |
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| Institution: | ZHANG Ji-yu1,ZHANG Zhen2,QIAO Yu-shan2,XUAN Ji-ping1,GUO Zhong-ren1* (1.Institute of Botany,Jiangsu Provincial and Chinese Academy of Sciences,Nanjing 210014,China,2.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Taking the total cDNA library of Hubei provincial Malus hupehensis treated by salicylic acid(SA) as templates,its PR10 multigene family was amplified by PCR technology,the sequences and their expression characteristics were analyzed by using bioinformatics method.The results revealed that 16 PR10 gene sequences were isolated,their coding regions all were 477 bp in length,encoding 159 amino acids,their molecular weights were from 17.53 to 17.85 kDa,and the isoelectric points were 5.06~6.40.These 16 genes wer... |
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| Keywords: | Hubei provincial Malus hupehensis MhPR10 gene family Characteristic |
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