| 经基因工程改造的花生主要过敏原Arah2制备及其低致敏原性鉴定 |
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| 引用本文： | 易海涛,刘芳,贾梦阳,汤慕瑾,曹小勇,夏立新,刘志刚.经基因工程改造的花生主要过敏原Arah2制备及其低致敏原性鉴定[J].江西农业大学学报,2011(5):993-998. |
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| 作者姓名： | 易海涛 刘芳 贾梦阳 汤慕瑾 曹小勇 夏立新 刘志刚 |
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| 作者单位： | 深圳大学生命科学学院;深圳大学医学院过敏反应与免疫学研究所;新疆华世丹药业股份有限公司;深圳大学医学院;深圳市出入境检验检疫局;福田区妇幼保健院; |
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| 基金项目： | 国家自然科学基金(30871752); 深圳出入境检验检疫局科技计划项目(SZ2008105); 深圳大学创新团队基金资助项目(200904) |
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| 摘 要： | 构建经基因工程改造的Ara h 2表达载体,表达并纯化该蛋白,鉴定其过敏原性.将花生主要过敏原Arah2基因序列进行颠换,并将颠换后的序列进行合成,再将合成后的基因克隆到原核表达载体pET -32a(+)上,然后转入Origami宿主表达菌中；利用Isopropyl β-D -1 - Thiogalactopyrano...
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| 关 键 词： | 基因工程 花生 过敏原 Ara h 2 低致敏 |
Preparation and Characterization of the Genetically Engineered Hypoallergenic Derivative of the Major Peanut Allergen Ara h 2 |
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| YI Hai-tao,LIU Fang,JIA Meng-yang,TANG Mu-jing,CAO Xiao-yong,XIA Li-xin,LIU Zhi-gang,.Preparation and Characterization of the Genetically Engineered Hypoallergenic Derivative of the Major Peanut Allergen Ara h 2[J].Acta Agriculturae Universitis Jiangxiensis,2011(5):993-998. |
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| Authors: | YI Hai-tao LIU Fang JIA Meng-yang TANG Mu-jing CAO Xiao-yong XIA Li-xin LIU Zhi-gang |
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| Institution: | YI Hai-tao1,2,LIU Fang3,JIA Meng-yang4,TANG Mu-jing5,CAO Xiao-yong6,XIA Li-xin2,4*,LIU Zhi-gang2,4*(1.College of Life Science,Shenzhen University,Shenzhen 518060,China,2.Institute of Allergy and Immunology,3.Xinjiang Huashidan Pharmaceutical Co.,Ltd,Wulumuqi 830000,4.Medical College,5.Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518045,6.Maternal and Child Health Hospital of Futian,Shenzhen 5180... |
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| Abstract: | The genetically engineered major allergen Ara h 2 was expressed and purified and the hypoallergenic of purified recombinant F-Ara h 2 protein was characterized.The Ara h 2 was reassembled and inserted into the expression vector pET-32a(+).The vector was transformed into Origami and the protein expression was induced by IPTG.Ni2+ Chelating affinity chromatography was used to purify the recombinant F-Ara h 2 protein.The hypoallergenic of F-Ara h 2 was examined by Western blotting and ELISA.The ORF which conta... |
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| Keywords: | genetic engineering peanut allergen Ara h 2 hypoallergenic |
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