| 致病性大肠埃希氏菌脉冲场凝胶电泳图谱的建立 |
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| 引用本文： | 张媛.致病性大肠埃希氏菌脉冲场凝胶电泳图谱的建立[J].中国兽药杂志,2012,46(3):10-13. |
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| 作者姓名： | 张媛 |
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| 作者单位： | 中国兽医药品监察所,北京,100081 |
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| 摘 要： | 建立了致病性大肠埃希氏菌脉冲场凝胶电泳图谱。采用脉冲场凝胶电泳技术分离细菌DNA,EB染色,Info Quest FP聚类分析软件分析,结果获得了《中国兽医菌种目录》中收录的127株致病性大肠埃希氏菌脉冲场凝胶电泳图谱,其中CVCC212与CVCC213等12对大肠埃希氏菌脉冲场凝胶电泳图谱相似率在85%以上。实验通过建立127株致病性大肠埃希氏菌脉冲场凝胶电泳图谱,丰富了致病性大肠埃希氏菌背景资料,为进一步分析基因分型与致病性之间的关系提供了实验数据。
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| 关 键 词： | 大肠埃希氏菌 脉冲场凝胶电泳 染色体 |
| 收稿时间： | 2011/12/12 0:00:00 |
| 修稿时间： | 2011/12/20 0:00:00 |
Construction of Malgenic Escherichia coli's Pulsed-Field Gel Electrophoresis Map |
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| Zhang Yuan.Construction of Malgenic Escherichia coli's Pulsed-Field Gel Electrophoresis Map[J].Chinese Journal of Veterinary Drug,2012,46(3):10-13. |
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| Authors: | Zhang Yuan |
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| Institution: | China Institute of Veterinary Drug Control |
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| Abstract: | objective:construct pulsed-field gel electrophoresis maps of malgenic Escherichia coli.Method:segregated bacterial DNA used pulsed-field gel electrophoresis technology.Then marked with EB.Analyzed maps with Info Quest FP cluster analysis software in the end.Consequence:pulsed-field gel electrophoresis maps of 127 malgenic Escherichia coli strains included by CVCC Catalogue of Cultures were obtained,in which 12 strains including CVCC212 and CVCC213 were correlated to epidemiology of Escherichia coli.Conclusion:pulsed-field gel electrophoresis maps of 127 malgenic Escherichia coli strains constructed in the experiment consummated background information of malgenic Escherichia coli The experiment provided valuable data for analyzing further relationship between genotyping and pathogenicity. |
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| Keywords: | Escherichia coli Pulsed-Field Gel Electrophoresis Chromosome |
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