| 猪FBXL8基因第二内含子的克隆测序及SNP位点检测 |
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| 引用本文： | 李勇,杨述林,崔文涛,牟玉莲,唐中林,储明星,彭克美,李奎.猪FBXL8基因第二内含子的克隆测序及SNP位点检测[J].江西农业大学学报,2011(5):938-941. |
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| 作者姓名： | 李勇 杨述林 崔文涛 牟玉莲 唐中林 储明星 彭克美 李奎 |
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| 作者单位： | 江西农业大学动物科技学院;华中农业大学动物科学-动物医学院;中国农业科学院畜牧研究所家养动物遗传资源与种质创新重点开放实验室; |
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| 基金项目： | 国家自然科学基金项目(30571330、30700569); 江西省科技支撑计划项目和江西省农业厅项目 |
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| 摘 要： | 为探明猪FBXL8基因的分子结构特征,本试验以人FBXL8基因的mRNA序列作为信息探针,搜集猪同源基因EST序列,并构建EST重叠群Contig,设计出合适的引物,以湖北通城、大白和长白猪基因组为模板,应用PCR技术克隆测定FBXL8基因的第二内含子序列.序列分析结果表明猪FBXL8基因第二内含子为855 bp.通过...
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| 关 键 词： | FBXL8 第二内含子 克隆测序 SNP位点检测 |
Cloning,Sequencing and SNP Site Detection of the Second Intron of Porcine FBXL8 Gene |
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| LI Yong,,YANG Shu-lin,CUI Wen-tao,MU Yu-lian,TANG Zhong-lin,CHU Ming-xing,PENG Ke-mei,LI Kui.Cloning,Sequencing and SNP Site Detection of the Second Intron of Porcine FBXL8 Gene[J].Acta Agriculturae Universitis Jiangxiensis,2011(5):938-941. |
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| Authors: | LI Yong YANG Shu-lin CUI Wen-tao MU Yu-lian TANG Zhong-lin CHU Ming-xing PENG Ke-mei LI Kui |
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| Institution: | LI Yong1,2,3,YANG Shu-lin3,CUI Wen-tao3,MU Yu-lian3,TANG Zhong-lin3,CHU Ming-xing3,PENG Ke-mei2*,LI Kui3*(1.College of Animal Science and Veterinary Medicine,Jiangxi Agricultural University,Nanchang 330045,China,2.College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,3.The Key Laboratory for Domestic Animal Genetic Resources and Germplasm Innovation,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | In order to explore the molecular structural feature of porcine FBXL8 gene,the Homo FBXL8 mRNA sequence was used as an information probe to search for the porcine homologous ESTs sequence in this experiment,and the ESTs contig was constructed.Then the primers were designed according to it.Tongchen,Landrace and large white breeds DNA were used as the genomic template to amplify the second intron of porcine FBXL8 gene by PCR technique.The sequence analysis result indicates that it is a sequence of 855 bp long... |
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| Keywords: | FBXL8 intron 2 cloning and sequencing SNP site detection |
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