A simple and rapid method for the analysis of fish histamine by paper electrophoresis |
| |
| Authors: | Minoru Sato Zhi Hua Tao Kazuhiro Shiozaki Toshiki Nakano Toshiyasu Yamaguchi Takehiko Yokoyama Nobuhiro Kan-No Eizoh Nagahisa |
| |
| Affiliation: | Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, and;School of Fisheries Science, Kitasato University, Ofunato, Iwate 022-0101, Japan |
| |
| Abstract: | ABSTRACT: The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly's reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g). |
| |
| Keywords: | determination fish histamine paper electrophoresis Pauly reagent |
| 本文献已被 SpringerLink 等数据库收录！ |
|
