| 南方红豆杉内生真菌Bionectria sp.的分离、鉴定及代谢产物活性研究 |
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| 引用本文： | 余艳,胡昌华.南方红豆杉内生真菌Bionectria sp.的分离、鉴定及代谢产物活性研究[J].西南农业大学学报,2007,29(6):131-135. |
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| 作者姓名： | 余艳 胡昌华 |
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| 作者单位： | 西南大学药学院、现代生物医药研究所,重庆400716 |
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| 基金项目： | 重庆市科技攻关资助项目（2003-8110） |
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| 摘 要： | 从南方红豆杉树皮中分离到30株内生真菌,结合形态学和ITS-5.8S rDNA序列两种鉴定方法,确定其中1株（XH004）为生赤壳属（Bionectria）真菌,这是目前世界上首次从红豆杉科植物中分离到该属内生真菌.通过抑菌实验、细胞毒实验、TLC检测和HPCL检测证明XH004代谢产物具有明显的抗菌和杀细胞作用,且极可能含有紫杉醇或紫杉醇类似物.
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| 关 键 词： | 南方红豆杉 内生真菌 生赤壳属 紫杉醇 |
| 文章编号： | 1673-9868（2007）06-0131-05 |
| 修稿时间： | 2006-12-28 |
Separation and Identification of a New Taxus chinensis var. mairei Endophytic Fungus (Bionectria sp.)and the Activity of Its Metabolites |
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| YU Yan, HU Chang-hua.Separation and Identification of a New Taxus chinensis var. mairei Endophytic Fungus (Bionectria sp.)and the Activity of Its Metabolites[J].Journal of Southwest Agricultural University,2007,29(6):131-135. |
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| Authors: | YU Yan HU Chang-hua |
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| Institution: | Institute of Modern Biopharmaceutics , School of Pharmaceutical Sciences, Southwest University, Chongqing 400716 , China |
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| Abstract: | Thirty endophytic fungi have been isolated from the bark of Taxus chinensis var.mairei.Morphologic and ITS-5.8S rDNA sequence identification methods used in combination identified one of them(XH004)to be Bionectria sp.,which is a newly-recorded species in Taxaceae endophytic fungi.Bacteriostatic test,cytotoxic test,thin layer chromatography(TLC)and high performance liquid chromatography(HPLC)confirmed that the metabolites of XH004 had obvious bacteriostatic and cytocidal activities and suggested that they probably contain certain taxol or taxol analog. |
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| Keywords: | Taxus chinensis var mairei endophytic fungus Bionectria taxol |
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