共表达禽流感病毒HA和NA基因的重组禽痘病毒在SPF鸡的免疫效力试验

 将禽流感病毒A/Goose/Guangdong/3/96(H5N1)毒株的HA和NA2个基因同源重组到禽痘病毒基因组中,获得了能同时高效表达这2种蛋白的重组禽痘病毒(rFPV-HA-NA)。将rFPV-HA-NA经翅膀刺种途径接种8周龄SPF鸡,免疫后4周分别用10LD50的高致病力禽流感病毒(HPAIV)A/Goose/Guangdong/1/96(H5N1)和A/FPV/Rostock/34(H7N1)毒株进行攻击。结果重组禽痘病毒免疫鸡群诱导产生了高水平的抗体,能够完全抵抗H5N1和H7N1亚型病毒的

Immune Efficacy of a Recombinant Fowlpox Virus Co-expressing HAand NA Genes of Avian Influenza Virus in SPF Chickens

A recombinant fowlpox virus co-expressing haemagglutinin (HA) and neuraminidase (NA) named as rFPV-HA-NA was produced by HA and NA gene of A/Goose/Guangdong/3/96 (H5N1) isolate of avian influenza virus recombinedinto the genome of fowlpox virus. In this study, to evaluate its ability to protect chickens against challenge with a lethal doseof highly pathogenic isolates of avian influenza virus, eight-week-old specific-pathogenic-free (SPF) chickens were vaccinatedwith recombinant virus or the wildtype fowlpox virus by wing-web puncture. Following challenge 4 weeks later with 10LD50highly pathogenic avian influenza virus H5N1 and H7N1 isolate, all chickens vaccinated with recombinant virus wereprotected, while the chickens vaccinated with the wildtype fowlpox virus or unvaccinated controls experienced 100%mortality respectively following challenge. And this complete protection was accompanied by the high levels of specificantibody response to the respective components of the recombinant virus.