Inheritance of angular leaf spot resistance in common bean line BAT 332 and identification of RAPD markers linked to the resistance gene |
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Authors: | Eveline Teixeira Caixeta Aluízio Borém Samir de Azevedo Fagundes Silvia Niestche Everaldo Gonçalves de Barros Maurílio Alves Moreira |
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Institution: | 1. Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Vi?osa, 36570-000, Vi?osa, MG, Brazil 2. Dept. Fitotecnia, Universidade Federal de Vi?osa, 36570-000, Vi?osa, MG, Brazil 5. Unimontes, Janaúba, MG, Brazil 3. Dept. Biologia Geral/BIOAGRO, Universidade Federal de Vi?osa, 36570-000, Vi?osa, MG, Brazil 4. Depto Bioquímica e Biologia Molecular/BIOAGRO, Universidade Federal de Vi?osa, 36570-000, Vi?osa, MG, Brazil
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Abstract: | The existence of genetic variability for angular leaf spot (ALS) resistance in the common bean germplasm allows the development
of breeding lines resistant to this disease. The BAT 332 line is an important resistance source to common bean ALS. In this
work we determined the inheritance pattern and identified RAPD markers linked to a resistance gene present in BAT 332. Populations
F1, F2,BCs and BCr derived from crosses between BAT 332 and cultivar Rudá were used. Rudá is a commercial cultivar with carioca
type grains and susceptible to ALS. The resistance of BAT 332 to race 61.41 of the pathogen was confirmed. Segregation analysis
of the plants indicated that a single dominant gene confers resistance. For identification of RAPD markers linked to the resistance
gene, bulk segregant analysis (BSA) was used. Two RAPD markers,OPAA07950 and OPAO12950, linked in coupling phase at 5.10 and 5.83 cM of this gene, respectively, were identified. These molecular markers are important
for common bean breeders and geneticists as source of genetic information and for marker assisted selection in breeding programs.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | angular leaf spot Phaeoisariopsis griseola RAPD markers resistance inheritance |
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