淮南猪品种遗传特异性的AFLP分析

试验利用25对引物对河南省地方品种淮南猪及引入品种杜洛克猪、长白猪、大约克猪4个品种猪进行AFLP分析。电泳检测结果发现,25对引物共检测到317条扩增片段,其中多态性片段79条,占24.92%。多态性统计分析表明,长白猪与大约克猪之间的遗传距离指数为0.0070,遗传关系最近;淮南猪与杜洛克猪、大约克猪遗传距离指数相近,分别为0.0073、0.0075,遗传关系较近;淮南猪与长白猪遗传距离指数为0.0078,遗传关系最远。结果显示,河南省地方品种淮南猪相对于其他引入品种特异性强,具有很高的育种价值,同时也证明了AFLP技术可以很好地检测猪种群内的遗传变异及猪种鉴别。

AFLP Analysis of the Genetic Specificity of Huainan Pig

25 pairs of primers were used to analyse the Huainan pig which was the local variety in Henan province and the three foreign pigs of Duroc,Landrace,Yorkshire pigs with the AFLP method. With 25 pairs of primers,we detected 317 fragments in all,79 fragments were polymorphic,accounting for 24.92%. Statistical analysis showed that the genetic index between Landrace and Yorkshire pigs was 0.0070,which indicated that these two pigs had the closest genetic relationship, Huainan pig and Duroc,Yorkshire pig had the similar genetic distance index,which were 0.0073,0.0075,respectively. With the choser genetic relationship,the genetic distance index between Huainan pig and Landrace was 0.0078,genetic relationship between them was the farthest. The results showed that the Huainan pig had strong specificity,comparing with other introduced species,and with high breeding value,but also proved that AFLP technique could detect genetic variation and pig identification within the populations of pigs.