| 基于等位基因特异性PCR原理建立鸡白痢沙门氏菌PCR方法 |
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| 引用本文： | 涂玉蓉,陈建红,任涛,张济培,司兴奎,牛森.基于等位基因特异性PCR原理建立鸡白痢沙门氏菌PCR方法[J].中国畜牧兽医,2011,38(5):93-96. |
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| 作者姓名： | 涂玉蓉 陈建红 任涛 张济培 司兴奎 牛森 |
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| 作者单位： | 1. 佛山科学技术学院动物医学系,广东佛山 528231;2. 华南农业大学兽医学院,广东广州 510642 |
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| 摘 要： | 根据鸡白痢沙门氏菌与鸡伤寒沙门氏菌的rfbS基因在第237和598位碱基的不同,设计和合成等位基因特异性PCR引物,建立快速检测鸡白痢沙门氏菌的PCR方法,并应用该法对鸡白痢沙门氏菌临床分离样品进行了PCR鉴定。结果显示,该PCR方法能特异性地鉴定鸡白痢沙门氏菌,检测灵敏度达18 pg/μL DNA,4.7×104 CFU/mL菌液,表明建立的等位基因特异性PCR方法能准确而快速地鉴定鸡白痢沙门氏菌。
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| 关 键 词： | 等位基因特异PCR 鸡白痢沙门氏菌 rfbS基因 |
| 收稿时间： | 2010-11-09 |
Development PCR Assay for Detecting Salmonella pullorum Based on Allele-specific PCR |
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| TU Yu-rong,CHEN Jian-hong,REN Tao,ZHANG Ji-pei,SI Xing-kui,NIU Sen.Development PCR Assay for Detecting Salmonella pullorum Based on Allele-specific PCR[J].China Animal Husbandry & Veterinary Medicine,2011,38(5):93-96. |
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| Authors: | TU Yu-rong CHEN Jian-hong REN Tao ZHANG Ji-pei SI Xing-kui NIU Sen |
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| Institution: | 1. College of Life Science,Foshan University,Foshan 528231,China;2. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China |
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| Abstract: | Based on the nuclei polymorphism of rfbS gene sequence of Salmonella pullorum at site 237 and 598 compared with S.gallinarum,a pair of allele-specific primers were designed and synthesized,and an allele-specific PCR method for detecting S.pullorum was developed consistent with result of the serological and biochemical method and the sensitivity of the PCR assay was 18 pg DNA and 4.7×104 CFU/mL cultural liquid.The results showed that the developed allele-specific PCR was a very rapid,sensitive and specific molecular tool for the detection and identification of S.pullorum isolates. |
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| Keywords: | allele-specific PCR Salmonella pullorum rfbS gene |
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