| 猪防御素基因pBD-2在大肠杆菌中的重组和融合表达 |
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| 引用本文： | 徐海涛,马立保,何启盖,廖冰麟,任芬芬. 猪防御素基因pBD-2在大肠杆菌中的重组和融合表达[J]. 中国畜牧兽医, 2011, 38(5): 101-104 |
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| 作者姓名： | 徐海涛 马立保 何启盖 廖冰麟 任芬芬 |
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| 作者单位： | 1. 华中农业大学农业微生物学国家重点实验室,湖北武汉 430070;2. 华中农业大学动物科技学院,湖北武汉 430070;3. 湖南农业大学动物科学技术学院,湖南长沙 410128 |
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| 基金项目： | 国家生猪产业体系技术建业项目 |
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| 摘 要： | 哺乳动物防御素是动物机体在抵御病原微生物的防御反应中产生的一类重要的抗菌肽物质,具有十分广泛的抗菌谱,在先天免疫上起重要作用。本研究根据已报道的pBD-2基因cDNA序列,合成了3条基因片段(1、2、3)。片段1、2和片段2、3各有15个碱基互补配对,PCR扩增延伸得到pBD-2基因,将pBD-2基因克隆至原核表达载体pGEX-KG,成功地在大肠杆菌BL21(DE3)中表达出pBD-2融合蛋白。pBD-2基因的成功表达为进一步研究其抗菌活性打下了基础。
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| 关 键 词： | 猪β-防御素-2 原核表达 融合蛋白 |
| 收稿时间： | 2010-10-27 |
Recombination and Fusion Expression of Porcine Defensin Gene pBD-2 in E.Coli |
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| XU Hai-tao,MA Li-bao,HE Qi-gai,LIAO Bing-lin,REN Fen-fen. Recombination and Fusion Expression of Porcine Defensin Gene pBD-2 in E.Coli[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(5): 101-104 |
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| Authors: | XU Hai-tao MA Li-bao HE Qi-gai LIAO Bing-lin REN Fen-fen |
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| Affiliation: | 1. National Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China;2. College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;3. College of Animal Science and Technology,Hunan Agricultural University,Chansha 410128,China |
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| Abstract: | Defensin is an important antimicrobial peptide produced in the course of animals’ defense against pathogens,which has a broad antibacterial spectrum and plays an important role in innate immunity. Based on cDNA sequence of porcine defensin β-defensin-2 gene reported in this study,three gene fragment (1,2,3) were synthesized in this experiment. Fragment 1,2 and fragment 2,3 had respectively 15 bases for complementary pair. pBD-2 gene was produced by PCR amplifing,and pBD-2 was inserted into expression vector (pGEX-KG) and recombinant vector transformed into E.coli,where recombinant vector produced fusion protein successfully.The expression of pBD-2 gene lays a foundation in research on antimicrobial activities of defensin. |
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| Keywords: | porcine defensin β-defensin-2 prokaryotic expression fusion protein |
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