| 菜豆抗草甘膦基因SSR分子标记的研究 |
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| 引用本文： | 张俐俐,陶波.菜豆抗草甘膦基因SSR分子标记的研究[J].北方园艺,2009(10). |
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| 作者姓名： | 张俐俐 陶波 |
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| 作者单位： | 东北农业大学,黑龙江,哈尔滨,150030;黑龙江省农业科学院,生物技术研究所,黑尼江,哈尔滨,150086;东北农业大学,黑龙江,哈尔滨,150030 |
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| 基金项目： | 黑龙江省农业科学院2006年度重点研究资助项目 |
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| 摘 要： | 利用抗草甘膦亲本89-05-3与敏感草甘膦亲本89-13杂交后获得F2代群体,采用BSA法,以上述F2代为定位群体,利用抗、感亲本和抗、感池筛选10对SSR引物.结果袁明:3对引物BM143、BM156、BM164均能在抗、感池间扩增出多态性条带,用Mapmaker 3.0软件作图,将该抗草甘膦基因初步定位在引物BM156和BM164之间,遗传距离分别为3.3 cM和4.4 cM,并暂命名为EPSPS2.该研究结果为莱豆抗性育种以及抗草甘膦基因的精细定位和图位克隆奠定了基础.
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| 关 键 词： | 菜豆 抗草甘膦基因 SSR |
Study on SSR Molecular Markers of Kidney Bean Glyphosate Resistance Gene |
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| ZHANG Li-li,TAO Bo.Study on SSR Molecular Markers of Kidney Bean Glyphosate Resistance Gene[J].Northern Horticulture,2009(10). |
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| Authors: | ZHANG Li-li TAO Bo |
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| Institution: | ZHANG Li-li1,2,TAO Bo1(1.Northeast Agriedtural University,Harbin,Heilonjiang 150030,China,2.Biotechnology Institute Heilongjiang Academy of Agricultural Sciences,Heilonjiang 150086,China) |
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| Abstract: | In this paper,F2 generation groups were obtained by hybridization of anti-glyphosate parent 89-05-3 and sensitive glyphosate parent 89-13 hybrid,10 pairs of SSR primers were filtered by useing resistant and susceptible parents and resistant and susceptible pool on behalf of the above-mentioned F2 as target groups with BSA law.The results showed that 3 pairs of primers BM143,BM156,BM164 can all amplified polymorphic bands between resistant and susceptible pool,using the software Mapmaker 3.0 mapping,the glyp... |
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| Keywords: | SSR |
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