| 野蚕孵化酶基因的克隆与生物信息学分析 |
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| 引用本文： | 唐顺明,赵新慧,吴俊,裘智勇,沈兴家.野蚕孵化酶基因的克隆与生物信息学分析[J].江西农业学报,2011(10):1-5. |
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| 作者姓名： | 唐顺明 赵新慧 吴俊 裘智勇 沈兴家 |
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| 作者单位： | 江苏科技大学;中国农业科学院蚕业研究所农业部蚕桑遗传改良重点开放实验室; |
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| 基金项目： | 国家高技术研究发展计划863重点项目(2007AA100504); 江苏省自然基金项目(SBK200930217); 江苏省高校自然科学基金项目(08KJB230001) |
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| 摘 要： | 采用RT-PCR法从野蚕胚胎中克隆了一个885 bp孵化酶基因,命名为BmandHE(GenBank登录号:JN620366)。BmandHE ORF编码294个氨基酸残基,蛋白分子质量为33.57 kD,等电点为5.55。在BmandHE N-端存在16个氨基酸的信号肽,BmandHE序列含有锌指结合序列、Met-转角基序等孵化酶保守功能区域和构成孵化酶二级结构骨架的4个半胱氨酸残基。BmandHE与其他动物孵化酶氨基酸序列的相似性为30.4%～97.1%。进化分析表明,野蚕与昆虫家蚕、柞蚕、家蚊聚为一类,亲缘关系比较近。
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| 关 键 词： | 野蚕 孵化酶基因 ORF 克隆 生物信息学分析 |
Cloning and Bioinformatic Analysis of Hatching Enzyme Gene in Chinese Wild Silkworm(Bombyx mandarina) |
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| TANG Shun-ming,ZHAO Xin-hui,WU Jun,QIU Zhi-yong,SHEN Xing-jia.Cloning and Bioinformatic Analysis of Hatching Enzyme Gene in Chinese Wild Silkworm(Bombyx mandarina)[J].Acta Agriculturae Jiangxi,2011(10):1-5. |
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| Authors: | TANG Shun-ming ZHAO Xin-hui WU Jun QIU Zhi-yong SHEN Xing-jia |
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| Institution: | TANG Shun-ming1,2,ZHAO Xin-hui1,WU Jun1,QIU Zhi-yong1,SHEN Xing-jia1,2(1.Jiangsu University of Science & Technology,Zhenjiang 212003,China,2.Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Key Opening Laboratory of Genetic Improvement of Silkworm and Mulberry,Ministry of Agriculture,Zhenjiang 212018,China) |
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| Abstract: | By means of RT-PCR technology,we cloned a 885 bp(in length) hatching enzyme(HE) gene from the embryo of Chinese wild silkworm(Bombyx mandarina),and this gene was designated as BmandHE(GenBank accession: JN620366).BmandHE ORF encodes 294 amino acids residues with the predicted molecular weight of 33.57 kD and the isoelectric point of 5.55.The deduced BmandHE sequence had a signal peptide of 16 amino acids in N terminator.The BmandHE sequence contained zinc-binding domain and Met-turn domain,which are the cha... |
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| Keywords: | Bombyx mandarina Hatching enzyme gene ORF Cloning Bioinformatic analysis |
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