| STR分型技术在牛亲子鉴定中的应用实例 |
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| 引用本文： | 黄娅琳.STR分型技术在牛亲子鉴定中的应用实例[J].江西农业学报,2011(10):23-24. |
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| 作者姓名： | 黄娅琳 |
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| 作者单位： | 南京森林警察学院;国家林业局野生动植物刑事物证鉴定中心; |
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| 基金项目： | 科技部林业公益项目(201004094); 南京森林警察学院科研基金项目(QN201109) |
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| 摘 要： | 分别以争议小牛和嫌疑母牛的基因组总DNA为模板,运用STR复合扩增技术对TGLA227、BM2113、TGLA53、ETH10、SPS115、TGLA126、TGLA122、INRA23、ETH3、ETH225、BM1824共11个STR(短串联重复序列)基因座进行扩增,扩增产物经遗传分析仪检测,检测结果通过GeneM...
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| 关 键 词： | STR分型技术 亲子鉴定 牛 |
An Application Case of STR Loci Analysis in Parentage Identification of Cattle |
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| HUANG Ya-lin.An Application Case of STR Loci Analysis in Parentage Identification of Cattle[J].Acta Agriculturae Jiangxi,2011(10):23-24. |
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| Authors: | HUANG Ya-lin |
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| Institution: | HUANG Ya-lin1,2(1.Nanjing Forest Police College,Nanjing 210046,China,2.Wildlife Material Evidence Appraisal Center of State Forestry Administration,China) |
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| Abstract: | Taking the genomic DNA of disputable calf and suspicion cow as template respectively,the amplifications of eleven STR(short tandem repeat) loci(TGLA227,BM2113,TGLA53,ETH10,SPS115,TGLA126,TGLA122,INRA23,ETH3,ETH225 and BM1824) were carried out by using the multiplex amplification technique of STR,and the PCR products were further separated and analyzed in a genetic analyzer,then the detection results were analyzed by GeneMapper fragment analysis software.The result was that the probability of parentage betwe... |
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| Keywords: | STR loci analysis Parentage identification Cattle |
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