响应面法优化超临界CO2萃取金柑籽中柠檬苦素工艺条件的研究

优化超临界CO2萃取柠檬苦素的工艺条件，为金柑籽中柠檬苦素的开发提供理化依据。采用单因素试验研究夹带剂、夹带剂浓度、萃取温度、萃取时间、萃取压力和二氧化碳流量对柠檬苦素提取率的影响，在单因素试验的基础上，采用响应面分析法建立了二次多项式数学模型，并分析模型的有效性与各因素间的交互作用。在萃取压力为33 MPa，萃取温度为50℃，萃取时间为2.5 h的条件下，柠檬苦素提取率达3.98 g/kg。在最佳条件下得到实验结果与模型预测值吻合，说明所建立的模型是切实可行的。超临界CO2萃取技术的运用，有利于柠檬苦素提取的工业化推广和应用。

Technical Parameter Optimization for Supercritical Carbon Dioxide Extraction of Limonin from the Kumquat Seeds by Response Surface Methodology

Technical parameter for supercritical carbon dioxide extraction of limonin from kumquat seeds were optimized, it could provide theoretical basis for the development of limonin. The single-factor experiments were used to investigate the effects of different co-solvent, co-solvent concentration, temperature, time, pressure and CO2 flow rate on limonin extraction rate. Based on the single-factor experiment, a quadratic-multinomial-mathematical model was established by Response Surface Methodology （RSM）. The applicability of the model and interaction involved factors on extraction of limonin was verified. The results indicated that when the pressure of 33 MPa, temperature of 50℃, exaction time of 2.5 h, the highest exaction rate of limonin was 3.98 g/kg. Under the optimum condition, the results showed the model fitted well the experimental data and the model was feasible. The utilization of supercritical carbon dioxide extraction technology was beneficial to the industrialization promotion and application of limonin extraction.