| 稻瘟病菌大片段DNA转化载体的构建 |
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| 引用本文： | 陈亚平,施文骁,王洪凯. 稻瘟病菌大片段DNA转化载体的构建[J]. 中国水稻科学, 2015, 29(1): 91-96. DOI: 10.3969/j.issn.1001-7216.2015.01.011 |
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| 作者姓名： | 陈亚平 施文骁 王洪凯 |
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| 作者单位： | 浙江大学 农业与生物技术学院， 杭州 310058; |
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| 基金项目： | 国家自然科学基金资助项目，浙江省自然科学基金资助项目 |
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| 摘 要： | 构建能够转化大片段DNA的载体是进行基因簇功能分析的重要手段。利用cosmid载体和pBHt2载体构建了一个可以在稻瘟病菌中转化大片段DNA的载体,通过农杆菌介导的转化方法,成功地将超过30kb的大片段DNA转化到稻瘟病菌中。研究结果将促进稻瘟病菌基因簇的功能分析。
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| 关 键 词： | 载体 转化 大片段DNA 稻瘟病菌 |
| 收稿时间： | 2014-03-28; |
Construction of a Vector for Large DNA Fragment Transformation in Magnaporthe oryzae |
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| CHEN Ya ping,SHI Wen xiao,WANG Hong kai. Construction of a Vector for Large DNA Fragment Transformation in Magnaporthe oryzae[J]. Chinese Journal of Rice Science, 2015, 29(1): 91-96. DOI: 10.3969/j.issn.1001-7216.2015.01.011 |
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| Authors: | CHEN Ya ping SHI Wen xiao WANG Hong kai |
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| Affiliation: | College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; |
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| Abstract: | It is an important means to study function of gene cluster using special vectors for large DNA fragment transformation. A vector was constructed for large DNA fragment transformation based on cosmid super Cos I vector and pBHt2 vector. More than 30 kb DNA fragment was transferred into the rice blast fungus successfully using Agrobacterium tumefaciens mediated transformation. The results will promote functional analysis of gene cluster in Magnaporthe oryzae. |
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| Keywords: | vector transformation large DNA fragment Magnaporthe oryzae |
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