水稻新裂颖突变体sg1的遗传分析与基因定位

从中籼3037辐射突变体库中获得一个颖花开裂突变体(split glume1,sg1),表型分析发现该突变体不仅颖壳异常,且伴随着生育期提前,植株变矮,颖花退化等现象,结实率也显著降低。遗传分析和分子生物学分析结果表明该突变受1对隐性核基因控制,并最终将其定位在水稻第1染色体上分子标记M1-z21与M1-z27之间,物理距离约为47kb,这是一种控制颖花发育的新基因,通过预测发现该区段内存在6个开放阅读框。

Genetic Analysis and Fine Mapping for a Novel split glume Mutant in Rice

A split glume mutant  is from the mutant library of  indica  variety Zhongxian 3037 radiated by 60Co γ ray. Compared to its wild type, the mutant has not only split glumes, but also characterized by early heading, lower plant height, reduced seed setting rate and degenerated spikelets, which implies that the mutation has a pleiotropic effect. Genetic and molecular analysis indicated that the mutant was controlled by a recessive nuclear gene, temporally designated as split glume 1 (sg1), and then SG1 was fine mapped to a 47 kb DNA region between the STS markers M1 z21 and M1 z27 on 1, where six ORFs in total were predicted. On the basis of the location of SG1, it is deduced that SG1 is a novel gene regulating the rice glume development.