| 苹果离体叶片高效再生不定芽技术研究 |
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| 引用本文： | 师校欣,杜国强,高仪,马宝盤,肖建会,贾建航.苹果离体叶片高效再生不定芽技术研究[J].果树学报,1999,16(4):255-258. |
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| 作者姓名： | 师校欣 杜国强 高仪 马宝盤 肖建会 贾建航 |
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| 作者单位： | 1. 河北农业大学园艺系,保定,071001
2. 中国科学院遗传研究所 |
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| 基金项目： | 河北省自然科学基金，河北省教委博士基金 |
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| 摘 要： | 苹果不同品种叶片再生不定芽能力不同，嘎拉再生能力最强，其次是乔纳金，富士最难再生。用继代培养３ 年以上、当代培养３０ ～５０ ｄ 的试管苗，取顶部第２ ～４ 叶位幼嫩叶片，远轴面向下接触培养基，再生效率高。诱导叶片再生不定芽适宜培养基为ＭＳ＋ ＴＤＺ１．０ ｍｇ·ｌ－ １（ 富士、乔纳金）或ＢＡ５ ．０ ｍｇ·ｌ－ １ ＋ ＮＡＡ０ ．１ ～０．２ ｍｇ·ｌ－ １ ＋ 蔗糖３５ ．０ ｇ·ｌ－ １ ＋ 琼脂６ ．２ ｇ·ｌ－ １（ 嘎拉） 。
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| 关 键 词： | 苹果 离体叶片 再生 |
High Adventitious Shoot Regeneration from Leaves in vitro of Apple Cultivars |
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| Shi Xiaoxin,Du Guoqiang,Gao Yi,Ma Baokun,Xiao Jianhui,Jia Jianhang.High Adventitious Shoot Regeneration from Leaves in vitro of Apple Cultivars[J].Journal of Fruit Science,1999,16(4):255-258. |
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| Authors: | Shi Xiaoxin Du Guoqiang Gao Yi Ma Baokun Xiao Jianhui Jia Jianhang |
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| Abstract: | The regeneration efficiency of leaf is different among apple cultivars, the highest one was in Gala,followed Jonagold and Fuji.It could has a higher regeneration efficiency when the leaves selected from the plantlet,which was subcultured for over 3 years and cultured for 30～50 d and laid on the medium in abaxial layer downside.The optimal medium for regeneration is MS+TDZ 1.0 mg·l -1 (Fuji and Jonagold) or BA 5.0 mg·l -1 (Gala)+NAA 0.1 ～ 0.2 mg·l -1 +Sucrose 35.0 g·l -1 +Agar 6.2 g·l -1 . |
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| Keywords: | Apple Leaf in vitro Regeneration |
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