| 植物甜蛋白monellin基因的合成和克隆 |
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| 引用本文： | 李英,尹俊,张树军. 植物甜蛋白monellin基因的合成和克隆[J]. 内蒙古农业大学学报(自然科学版), 2001, 22(3): 35-38 |
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| 作者姓名： | 李英 尹俊 张树军 |
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| 作者单位： | 内蒙古农业大学生物工程系 |
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| 基金项目： | 内蒙古自治区科学基金资助 |
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| 摘 要： | 根据已报道的单链monellin基因序列，设计合成5段核苷酸序列，利用这些片段末端的碱基互补序列经链延伸、PCR扩增合成双链DNA，克五笔型于质粒载体pUC18的BamHI、SalI位点之间，经限制酶切分析，序列分析等方法证实获得了monellin基因的克隆，与报道的monellin基因的序列完全符合，为下一步构建高效表达质粒，并将monellin基因转入植物并获得高含量的monellin转基因植物奠定基础。
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| 关 键 词： | monellin基因 合成 克隆 序列分析 植物甜蛋白 |
| 文章编号： | 1009-3575(2001)03-0035-04 |
| 修稿时间： | 2001-04-27 |
SYHTHESIS AND CLONING OF cDNA ENCODING THE SWEET PLANT PROTEIN MONELLIN |
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| LI Ying,YIN Jun,ZHANG Shu-jun. SYHTHESIS AND CLONING OF cDNA ENCODING THE SWEET PLANT PROTEIN MONELLIN[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2001, 22(3): 35-38 |
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| Authors: | LI Ying YIN Jun ZHANG Shu-jun |
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| Abstract: | Five specific primers were designed according to genomic sequence of monellin reported in literature.The DNA was synthesized by chain extension,followed by PCR amplification.The synthesized DNA was cloned into plasmid pUC18 in E.coli JM 109.The recombinant plasmid was screened and further identified by restriction enzyme analysis and nucleotide sequence analysis.The results showed that the sequence of the cloning is same as that of monellin reported in literature. |
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| Keywords: | monellin synthesis cloning |
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