| 不结球白菜子叶离体培养再生植株 |
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| 引用本文： | 侯喜林,曹寿椿. 不结球白菜子叶离体培养再生植株[J]. 南京农业大学学报, 1994, 0(3) |
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| 作者姓名： | 侯喜林 曹寿椿 |
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| 作者单位： | 南京农业大学园艺学系 |
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| 摘 要： | 用矮脚黄的子叶建立了不结球白菜高频率再生体系。在ＭＳ＋６－ＢＡ１０ｍｇ／Ｌ＋ＮＡＡ０．３ｍｇ／Ｌ培养基上，外植体分化率达７０％，每一外植体可产生９．６个不定芽。研究了各种因素对子时离体再生植株的影响，发现１１～１５ｄ苗龄的子叶分化效果最佳。ＭＳ＋ＮＡＡ０．２ｍｇ／Ｌ对根的诱导最好。
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| 关 键 词： | 不结球白菜；子叶；离体培养；再生植株 |
IN VITRO PLANT REGENERATION FROM COTYLEDON CULTURE OF NON-HEADING CHINESE CABBAGE |
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| Hou Xilin and Cao Shouchun. IN VITRO PLANT REGENERATION FROM COTYLEDON CULTURE OF NON-HEADING CHINESE CABBAGE[J]. Journal of Nanjing Agricultural University, 1994, 0(3) |
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| Authors: | Hou Xilin and Cao Shouchun |
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| Abstract: | The cotyledon of Brassica campestris ssp.chinensis Makino cultivar Aijiaohuang has been used to institute high capacity of regeneration.The shoot-bud differentiation frequency reached as high as 70% and each explant produced up to 9.6 adventitious buds on MS 6-BA 10 mg/L NAA 0.3 mg/L.Effects of various factors on shoot-bud differentiation in vitro,were investigated.The experimental results showed that the seedling age was very important,i.e.the cotyledon explants taken from 11-5-day-old seedlings displayed the highest shoot-bud regeneration capacity.The medium of MS NAA 0.2 mg/L had the best inducing effect on root formation. |
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| Keywords: | Brassica campestris ssp.chinensis Makino cotyledon culture in vitro plant regeneration |
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