导入外源DNA小麦蛋白质变异性的研究

ＳＤＳ－ＰＡＧＥ分析外源蜿豆花ＤＮＡ直接导入小麦体的种子蛋白质，结果表明：变异系小麦种子蛋白质组分出出现广泛变化，小偃６号和超大穗小麦分别增加４７ＫＤ，７１ＫＤ和９０ＫＤ，８５ＫＤ蛋白质新组分，蛋白质含量也相应增加约２２％和３１％，“中国春”小麦却出现了９２ＫＤ，８２ＫＤ蛋白质组分消失现象，其蛋白质含量也稍有降低，这此变异现象在后代中亦然表达，因此基因直接转移技术是优质分子育种和品质改良的新途径。

Variation of Seed Protein in Wheat Grain after Introducing DNA from Pea Flower

The seed proteins of variant lines of wheat into which exogenous DAN was directly introduced were analyzed via SDS PAGE.The result showed that the seed protein components remarkbly changed:in Xiaoyan 6 and Suporear wheat varieties the new proteins such as 47KD,71KD and 90KD,85KD appeared,and the protein contents also increased about 22% and 31% compared with that of the control.On the other hand,in Chinese Spring wheat protein components 92KD,82KD disappeared,and the protein contents decreased slightly (6%).These variations can be stably passed onto the progenies.It is,therefore,suggected that the technology of direct exogenous gene transfer to plants may be a new approach for breeding and improving nutrient quality in wheat.