微波处理构建不感染内生真菌德兰臭草种群的方法探讨

微波技术会对生物组织产生一定的生物学效应。为了研究微波技术对德兰臭草(Melica transsilvanica)内生真菌的去除效果,采用微波中低档对感染内生真菌的德兰臭草种子进行不同时间的杀菌处理,通过显微镜检结合植株内生真菌分离技术、种子萌发指标、植株生长指标评价内生真菌去除效果。结果表明,输出功率300 W的微波处理30s能有效杀灭种子中的内生真菌,并对种子发芽率、发芽势和发芽指数均无显著影响;微波处理10s,20s分别降低植株带菌率的10.7%,25%,并且对植株生长有促进作用;微波处理较长时间(40s及以上时间)虽能有效杀灭种子中的内生真菌,但对种子萌发、植株生长有抑制作用。因此,微波处理种子技术是一种有效、简便、可行的内生真菌去除技术。

Using Microwave Treatment to Construct Endophyte-free Melica transsilvanica Population

In this study mid-low microwave level treatment was used to construct endophyte-free populations of Melica transsilvanica.The elimination effects of the microwave on endophyte were tested by microscope test combined with Endophyte separation,seeds germination index and plant growth index.The result showed that 30 s microwave treatment was effective in killing the endophyte in the seeds.At the same time,there were no obvious adverse effects on the seeds germination rate,germinating potential and germination index.10 s and 20 s treatment in microwave could promote plant growth and decrease endophyte infection rate by 10.7％ and 25％,respectively.However,longer time treatment,40 s and more treatment time could inhibit the growth of plant.Therefore,microwave method was an effective way to construct endophyte-free populations of Melica transsilvanica.