| 木本植物叶片蛋白质双向电泳技术体系的建立 |
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| 引用本文： | 刘建,周坚.木本植物叶片蛋白质双向电泳技术体系的建立[J].江西农业大学学报,2006,28(5):758-761. |
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| 作者姓名： | 刘建 周坚 |
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| 作者单位： | 1. 南京林业大学,森林资源与环境学院,江苏,南京,210037;广西林业科学研究院,中南速生材繁育实验室,广西,南宁,530001
2. 南京林业大学,森林资源与环境学院,江苏,南京,210037 |
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| 基金项目： | 国家科技部“863”项目(2002AA241051)，广西林业科学研究院青年科技基金项目(2005f01) |
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| 摘 要： | 以2种木本植物的成熟叶片为材料,对传统的双向电泳进行改进和优化,得到1种适合于木本植物叶片蛋白质电泳分析的方法,此方法大大降低了木本植物叶片中次生代谢物质对双向电泳结果的干扰,明显改善了分析效果。
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| 关 键 词： | 木本植物 叶片蛋白质 双向电泳 |
| 文章编号： | 1000-2286（2006）05-0758-04 |
| 收稿时间： | 2005-12-25 |
| 修稿时间： | 2006-06-23 |
Establishment of Two-dimensional Gel Electrophoresis of Proteins from Leaf of Woody Plant |
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| LIU Jian,ZHOU Jian.Establishment of Two-dimensional Gel Electrophoresis of Proteins from Leaf of Woody Plant[J].Acta Agriculturae Universitis Jiangxiensis,2006,28(5):758-761. |
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| Authors: | LIU Jian ZHOU Jian |
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| Institution: | 1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China;2. Laboratory of Fast - Growing Tree Breeding and Cultivation in Central South China, Guangxi Forestry Research Institute, Nanning 530001, China |
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| Abstract: | Based on the method of O'farrell,an improved procedure of the two dimensional electrophoresis for analyzing leaf protein in woody plant which would greatly reduce the interference of the secondary metabolite of woody plants is presented.Some technical problems in the experiment are discussed in this article. |
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| Keywords: | woody plant leaf protein two-dimensional electrophoresis |
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