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| 茄子高频再生体系的建立与优化 | |
| 引用本文： | 李烨,金丹丹,谢立波,李景富.茄子高频再生体系的建立与优化[J].黑龙江农业科学,2014(9):48-52. |
| 作者姓名： | 李烨 金丹丹 谢立波 李景富 |
| 作者单位： | 1. 哈尔滨市农业科学院,黑龙江 哈尔滨,150001 2. 东北农业大学,黑龙江 哈尔滨,150030 3. 黑龙江省农业科学院,黑龙江哈尔滨,150086 |
| 摘 要： | 为建立并优化茄子高频再生体系,研究以茄子品种哈农杂茄2号和哈农杂茄3号为材料,利用不同的外植体和植物生长调节剂组合,探讨影响茄子再生频率的主要因素。结果表明:茄子最佳再生子叶最适分化培养基为MS 0+6-BA 1mg·L-1+KT 2mg·L-1;下胚轴最适分化培养基为MS 0+KT 2mg·L-1,再生苗最佳生根培养基为1/2MS+IAA 0.1mg·L-1,且下胚轴的分化效率高于子叶。 |
| 关 键 词： | 茄子 外植体 再生 |
Establishment and Optimization of High-f requency Regeneration System of Eggplant |
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| LI Ye,JIN Dan-dan,XIE Li-bo,LI Jing-fu.Establishment and Optimization of High-f requency Regeneration System of Eggplant[J].Heilongjiang Agricultural Science,2014(9):48-52. | |
| Authors: | LI Ye JIN Dan-dan XIE Li-bo LI Jing-fu |
| Institution: | LI Ye, JIN Dan-dan, XIE Li-bo, LI Jing-fu (1. Harbin Academy of Agriculture Sciences, Harbin, Heilongjiang 150001 ; 2. Northeast Ag- riculture University, Harbin, Heilongjiang 150030; 3. Heilongjiang Academy of Agriculture Sciences, Harbin, Heilongjiang 150023) |
| Abstract: | In order to establish and optimize the high-frequency regeneration system of eggplant ,taking cotyle-donary petiole and hypocotyl explants of Hanongzaqie 2 and Hanongzaqie 3 as materials ,the shoot regeneration frequencies of two eggplant（Solanum melongena L）cultivars were examined to discuss the factors affecting re-generation rate .The results showed that the optimum shoot inducing mediums for cotyledons was MS 0＋6-BA 1 mg·L-1 ＋KT 2 mg·L-1 ;the optimum shoot inducing mediums for hypocotyls was MS 0＋ KT 2 mg·L-1 ,the best rooting medium for regeneration seedling was 1/2MS＋IAA 0 .1 mg·L-1 .And the differentiation of hypo-cotyls was higher than that of cotyledons . |
| Keywords: | eggplant explants regeneration |
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