玉米SSR分子标记试验体系优化及其遗传多样性研究

为优化玉米SSR分子标记体系,丰富种质资源的多样性,以基础玉米自交系CK及其诱变系M为材料,优化了SSR分子标记试验体系,并对诱变玉米自交系遗传多样性进行了分析。结果表明:(1)优化后SSR分子标记试验体系,可以有效提高试验效率,减少试验误差;(2)利用23对SSR引物对诱变玉米自交系进行PCR扩增,共扩增得到59个等位基因,平均每对引物检测到2.565个等位基因;位点的多态信息量(PIC)和基因型多样性指数变化范围为0.110~0.525和0.234~1.061,平均值分别为0.331和0.643,说明基础材料CK与诱变材料存在真实的遗传差异。

System Optimization on Maize SSR Molecular Marker and Research of Genetic Diversity

In order to optimize the SSR molecular marker system and rich the diversity of germplasm resources , taking maize inbred line CK and its seven mutants as materials ,the experiment system was optimized and the genetic difference between mutants and the corresponding basic material was studied based on SSR markers with selected 23 pairs of polymorphism SSR primers .The results showed that（1）The optimization of SSR mo-lecular marker experiment system could effectively improve the efficiency of the experiment ,reduce the experi-mental error ;（2）By using the 23 pairs of SSR primer ,a total of 59 polymorphic amplified fragments was pro-duced .The average number of allele per SSR locus was 2 .565 .The polymorphism information content（PIC）for the SSR loci varied from 0 .110 to 0 .525 with an average of 0 .331 .The amplitude of genetic diversity indexes ranged from 0 .234 to 1 .061 with an average of 0 .643 .The phenomenon indicated that massive genetic variation could appear in maize（CK）progenies due to mutagenesis .