bFGF和IGF-1细胞生长因子对欧洲鳗鲡胸鳍传代细胞生长的影响

为建立一种欧洲鳗鲡病毒性疾病分离、鉴定的细胞模型,深入了解鳗鲡病毒性疾病流行与传播的情况,本试验在欧洲鳗鲡胸鳍传代细胞体外培养的最适条件下,通过添加碱性成纤维细胞生长因子(bFGF)和胰岛素样生长因子1(IGF-1)等可能的传代细胞促长因子,并在限定的时间内收集细胞、进行细胞计数、绘制胸鳍细胞的生长曲线,分析其对欧洲鳗鲡胸鳍细胞生长速率的影响。试验结果提示:在10%FBS L-15全价培养基的条件下,bFGF对欧洲胸鳍细胞的生长有明显的促进作用,最佳效应浓度为10μ.L-1;IGF-1(5～50μg.L-1)不利于欧洲鳗鲡胸鳍细胞的生长。

Growth Factors,bFGF and IFG-1,in Pectoral Fin Passage Cells of Anguilla anguilla

To establish a model for cell isolation and identification,as well as to gain an in-depth understanding of the viral diseases in Anguilla anguilla,the roles played by the growth factors,bFGF and IFG-1,on the proliferation of the eel’s pectoral fin cells were studied.Under in vitro conditions,the cells were collected,counted and growth curves constructed.On the L-15 medium containing 10% FBS,bFGF showed a significant evidence in promoting the growth of the pectoral fin cells.The cellular growth peaked at bFGF concentration of 10 μg·L-1.On the other hand,IGF-1 in the concentrations from 5 μg·L-1 to 50 μg·L-1 did not show any apparent effect on the growth.Therefore,a culture medium formulation for effective proliferation of the A.anguilla pectoral fin cells was obtained.This would,in turn,aid future studies on the pathogen′s isolation,identification and vaccine preparation.