| 我国部分小麦地方品种Waxy基因多样性研究 |
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| 引用本文： | 杜小燕,郝晨阳,张学勇,马正强,游光霞,王兰芬,董玉琛. 我国部分小麦地方品种Waxy基因多样性研究[J]. 作物学报, 2007, 33(3): 503-506 |
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| 作者姓名： | 杜小燕 郝晨阳 张学勇 马正强 游光霞 王兰芬 董玉琛 |
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| 作者单位： | 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物品种资源与生物技术重点开放实验室，北京100081;
南京农业大学作物遗传与种质创新国家重点实验室应用基因组学分室，江苏南京210095
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| 摘 要： | 利用2对STS引物、1对SSR引物和1对基因特异引物分析了1 739份中国小麦地方品种Waxy基因的变异情况。检测出Wx-A1基因缺失突变材料3份，Wx-B1基因缺失突变材料25份，Wx-D1基因缺失突变材料3份。利用SDS-PAGE方法对以上Waxy基因突变材料在蛋白质水平上进行鉴定，验证了Waxy分子标记在种质资源研究中的有效性。向育种家推荐一批具有不同Waxy基因缺失的地方种质，并提供了全部品种的缺失类型。
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| 关 键 词： | Waxy基因 小麦地方品种 分子标记 SDS-PAGE |
| 收稿时间： | 2006-04-12 |
| 修稿时间： | 1900-01-01 |
Genetic Diversity of Waxy Gene in Chinese Wheat Landraces |
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| Affiliation: | Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture/ National Key Facility for Crop Gene Resources and Genetic Improvement/ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; Applied Genomics Unit, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China |
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| Abstract: | A total of 1 739 Chinese wheat landraces were screened for the Waxy gene mutations with two STS, one SSR and one gene specific marker published in previous researches. Among of the landraces, 3 were null Wx-A1 mutants, 25 were null Wx-B1 mutants, and 3 were null Wx-D1 mutants. Different null types of Waxy genes were also certified by SDS-PAGE of waxy proteins, containing that these markers were effective to verify mutant and normal alleles at waxy loci. These markers can also be used in marker-assisted selection in breeding program. Furthermore, based on our findings, we recommend some accessions with different Waxy mutations to breeders. Genotypes of Waxy genes of these materials were also released in this paper. |
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| Keywords: | Waxy gene Wheat Landrace Molecular marker SDS-PAGE |
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